Genome-Wide Identification and Expression Analysis of the MADS Gene Family in Tulips (Tulipa gesneriana)

Jiaojiao Lu; Lianwei Qu; Guimei Xing; Zhenlei Liu; Xiaochun Lu; Xiaori Han

doi:10.3390/genes14101974

Genome-Wide Identification and Expression Analysis of the MADS Gene Family in Tulips (Tulipa gesneriana)

Genes (Basel). 2023 Oct 22;14(10):1974. doi: 10.3390/genes14101974.

Authors

Jiaojiao Lu^{1

2}, Lianwei Qu¹, Guimei Xing¹, Zhenlei Liu¹, Xiaochun Lu¹, Xiaori Han²

Affiliations

¹ Liaoning Academy of Agriculture Sciences, Shenyang 110161, China.
² College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China.

Abstract

To investigate the cold response mechanism and low temperature regulation of flowering in tulips, this study identified 32 MADS-box transcription factor family members in tulips based on full-length transcriptome sequencing, named TgMADS1-TgMADS32. Phylogenetic analysis revealed that these genes can be divided into two classes: type I and type II. Structural analysis showed that TgMADS genes from different subfamilies have a similar distribution of conserved motifs. Quantitative real-time PCR results demonstrated that some TgMADS genes (e.g., TgMADS3, TgMADS15, TgMADS16, and TgMADS19) were significantly upregulated in buds and stems under cold conditions, implying their potential involvement in the cold response of tulips. In summary, this study systematically identified MADS family members in tulips and elucidated their evolutionary relationships, gene structures, and cold-responsive expression patterns, laying the foundation for further elucidating the roles of these transcription factors in flowering and the cold adaptability of tulips.

Keywords: MADS; cold response; gene expression; phylogeny; tulip.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genome, Plant
MADS Domain Proteins / metabolism
Phylogeny
Transcription Factors / genetics
Tulipa* / genetics
Tulipa* / metabolism

Substances

MADS Domain Proteins
Transcription Factors

Grants and funding

This research was funded by the Program of China Agriculture Research System (CARS-23-G57), Subject Construction Program of Liaoning Academy of Agricultural Sciences (2022DD051608), and Presidential Foundation of Liaoning Academy of Agricultural Sciences (2023QN2403).