Cytotoxic Indole Diterpenoids from a Sphagneticola trilobata-Derived Fungus Aspergillus sp. PQJ-1

Wenxing Li; Guohui Yi; Kaiwen Lin; Guangying Chen; Yang Hui; Wenhao Chen

doi:10.3390/molecules28207003

Cytotoxic Indole Diterpenoids from a Sphagneticola trilobata-Derived Fungus Aspergillus sp. PQJ-1

Molecules. 2023 Oct 10;28(20):7003. doi: 10.3390/molecules28207003.

Authors

Wenxing Li^{1

2}, Guohui Yi³, Kaiwen Lin⁴, Guangying Chen^{1

2}, Yang Hui^{1

2}, Wenhao Chen^{1

2}

Affiliations

¹ Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China.
² Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China.
³ Public Research Center, Hainan Medical University, Haikou 571199, China.
⁴ Hainan Women and Children's Medical Center, Haikou 571158, China.

Abstract

Two new indole diterpene derivatives, 5S-hydroxy-β-aflatrem (1) and 14R-hydroxy-β-aflatrem (2), along with one known analogue, 14-(N,N-dimethl-L-valyloxy)paspalinine (3), were isolated from the fermentation broth of the fungus Aspergillus sp. PQJ-1 derived from Sphagneticola trilobata. The structures of the new compounds were elucidated from spectroscopic data and ECD spectroscopic analyses. All the compounds (1-3) were evaluated for their cytotoxicity against A549, Hela, Hep G2, and MCF-7 cell lines. Compounds 1 and 2 exhibited selective inhibition against Hela cells. Further studies showed that 1 significantly induced apoptosis and suppressed migration and invasion in Hela cells. Moreover, 1 could up-regulate pro-apoptotic genes BAX and Caspase-3 and down-regulate anti-apoptotic genes Bcl-xL and XIXP.

Keywords: Aspergillus sp. PQJ-1; Sphagneticola trilobata; cytotoxic activity; secondary metabolites.

MeSH terms

Antineoplastic Agents* / pharmacology
Aspergillus / chemistry
Asteraceae*
Diterpenes* / chemistry
Fungi
HeLa Cells
Humans
Indoles / chemistry
Molecular Structure

Substances

Antineoplastic Agents
Indoles
Diterpenes

Grants and funding

ZDYF2021SHFZ221/the Key Project of Research and Development of Hainan Province