Effects of different cryopreservation methods on canine isolated preantral follicles

Bence Somoskői; Lilla Bordás; Fusa Uno; Dóra Kispál; Linda Müller; Dóra Török; Sándor Cseh

doi:10.1016/j.anireprosci.2023.107361

Effects of different cryopreservation methods on canine isolated preantral follicles

Anim Reprod Sci. 2023 Nov:258:107361. doi: 10.1016/j.anireprosci.2023.107361. Epub 2023 Oct 20.

Authors

Bence Somoskői¹, Lilla Bordás², Fusa Uno³, Dóra Kispál⁴, Linda Müller⁵, Dóra Török⁶, Sándor Cseh⁷

Affiliations

¹ Department of Obstetrics and Food Animal Clinic, University of Veterinary Medicine, H-1078 Istvan str. 2, Budapest, Hungary. Electronic address: somoskoi.bence@univet.hu.
² Department of Obstetrics and Food Animal Clinic, University of Veterinary Medicine, H-1078 Istvan str. 2, Budapest, Hungary. Electronic address: bordas.lilla@univet.hu.
³ Department of Obstetrics and Food Animal Clinic, University of Veterinary Medicine, H-1078 Istvan str. 2, Budapest, Hungary. Electronic address: fusa.uno@student.univet.hu.
⁴ Department of Obstetrics and Food Animal Clinic, University of Veterinary Medicine, H-1078 Istvan str. 2, Budapest, Hungary. Electronic address: kispal.dora@univet.hu.
⁵ Department of Obstetrics and Food Animal Clinic, University of Veterinary Medicine, H-1078 Istvan str. 2, Budapest, Hungary. Electronic address: muller.linda@univet.hu.
⁶ Department of Obstetrics and Food Animal Clinic, University of Veterinary Medicine, H-1078 Istvan str. 2, Budapest, Hungary. Electronic address: torok.dora@univet.hu.
⁷ Department of Obstetrics and Food Animal Clinic, University of Veterinary Medicine, H-1078 Istvan str. 2, Budapest, Hungary. Electronic address: cseh.sandor@univet.hu.

PMID: 37890201
DOI: 10.1016/j.anireprosci.2023.107361

Abstract

The aim of the present study was to compare the survival and developmental rate of canine isolated preantral follicles (PAFs) after cryopreservation with different methods (closed vs open vitrification). Follicles were isolated from ovaries randomly divided into three groups: fresh control, OPS (open pulled straw) vitrified and cryotube (CT) vitrified. Post-thaw viability of follicles and oocytes was assessed. Fresh and vitrified/thawed PAFs were cultured in 20 µl drops of FSH-supplemented medium for 10 days. Follicular growth, survival rate, estradiol production and ovulation rate were examined. CT method resulted in lower rate of live cells (58.7%) and oocytes (38.8%) than that of fresh ones (83.6% and 64%, respectively) and OPS (80.3% and 79.3%, respectively). Survival rate was similar to fresh follicles in OPS group (98.5% and 95.4%, respectively), while CT decreased the survival to 81.2%. Fresh follicles showed continuous growth, while CT follicles stopped to increase their size after 2 day. In the OPS vitrified follicles, this halting occurred between Day5 and Day10. Fresh follicles showed the highest estradiol production (range: 26.9 - 266.2 pg/ml). Comparing the two vitrified groups, lower estradiol concentration range was measured in the CT group (7.8-48.7 pg/ml vs. 15.4-89.6 pg/ml). Ovulation rate in each group was lowest in the OPS group (1.7% vs 7% and 8.9% in fesh and CT, respectively). Our data show that OPS vitrification provides superior survival rate, in vitro growth and hormonal production to CT. To our knowledge, these are the first results on comparing different cryopreservation protocols on canine isolated preantral follicles.

Keywords: Canine; Cryopreservation; Preantral follicle; Vitrification.

Publication types

Randomized Controlled Trial, Veterinary

MeSH terms

Animals
Cryopreservation* / methods
Cryopreservation* / veterinary
Dogs
Estradiol / pharmacology
Female
Oocytes
Ovarian Follicle*
Ovary
Vitrification

Substances

Estradiol