Effect of Tangshen formula on the remodeling of small intestine and colon in Zucker diabetic fatty rats

Xin Yang; Jingbo Zhao; Hong Li; Lin Pan; Jing Guo; Jing Li; Yuting Zhang; Pengmin Chen; Ping Li

doi:10.1016/j.heliyon.2023.e21007

Effect of Tangshen formula on the remodeling of small intestine and colon in Zucker diabetic fatty rats

Heliyon. 2023 Oct 16;9(10):e21007. doi: 10.1016/j.heliyon.2023.e21007. eCollection 2023 Oct.

Authors

Xin Yang¹, Jingbo Zhao², Hong Li¹, Lin Pan¹, Jing Guo¹, Jing Li¹, Yuting Zhang¹, Pengmin Chen¹, Ping Li¹

Affiliations

¹ Beijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing (100029), China.
² Anbiping (Chongqing) Pathological Diagnosis Center, Chongqing, China.

Abstract

Background and aim: Previous study have demonstrated that Tangshen Formula (TSF) could attenuate colonic histomorphological remodeling in the diabetic rat model induced by high fat diet plus low dosage streptozotocin (STZ). However, it is not clear whether TSF has same effect on small intestine and the effect on biomechanical properties of bowel. The aim of this study is to investigate the effect of TSF on histomorphological and biomechanical remodeling of small intestine and colon by using Zucker Diabetic Fatty (ZDF) Rat model.

Materials and methods: ZDF rats (obese fa/fa) with blood glucose higher than 11.7 mmol/L were divided into ZDF group (diabetic control group) and ZDF + TSF group (TSF treatment group), the later were intragastrically administered TSF. The ZDF rats (lean fa/+) were served as normal control (ZL) group. The rats in the ZL and ZDF groups were administered with saline. The experimental period covered from 8 weeks to 24 weeks. At the end of experiment, the ileal and colonic segments were studied in vitro. The histomorphometry and biomechanical parameters were measured.

Results: Compared with ZL group histomorphologically, the wet weight per unit length, wall thickness, wall area and fractions of total and type I and type III collagen in different layers for both ileum and colon increased in ZDF group. Those increasing parameters were partially inhibited in ZDF + TSF group. Compared with ZL group biomechanically, ZDF and ZDF + TSF groups had smaller opening angle and residual strain in ileum, and bigger opening angle and residual strain in colon. Whereas the wall became softer in circumferential direction and stiffer in longitudinal direction for both ileum and colon. However, no difference of biomechanical parameters was found between ZDF and ZDF + TSF groups.

Conclusion: The histomorphological and biomechanical remodeling of ileum and colon were happened in ZDF rats （obese fa/fa）. TSF could partly attenuate ileal and colonic histomorphological remodeling rather than biomechanical remodeling.

Keywords: Diabetes; Gut remodeling; Tangshen formula; ZDF rat.