Сytotoxic effect of CAR-T cells against modified MCF-7 breast cancer cell line

Aigul Kh Valiullina; Ekaterina A Zmievskaya; Irina A Ganeeva; Margarita N Zhuravleva; Ekaterina E Garanina; Albert A Rizvanov; Alexey V Petukhov; Emil R Bulatov

doi:10.22099/mbrc.2023.47125.1820

Сytotoxic effect of CAR-T cells against modified MCF-7 breast cancer cell line

Mol Biol Res Commun. 2023;12(4):139-148. doi: 10.22099/mbrc.2023.47125.1820.

Authors

Aigul Kh Valiullina¹, Ekaterina A Zmievskaya¹, Irina A Ganeeva¹, Margarita N Zhuravleva¹, Ekaterina E Garanina¹, Albert A Rizvanov¹, Alexey V Petukhov², Emil R Bulatov^{1

3}

Affiliations

¹ Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia.
² Institute of Hematology, Almazov National Medical Research Center, 197341 Saint Petersburg, Russia.
³ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.

Abstract

The most often diagnosed and fatal malignancy in women is breast cancer. The International Agency for Research on Cancer (IARC) estimates that there are 2.26 million new cases of cancer in 2020. Adoptive cell therapy using T cells with chimeric antigen receptor shows potential for the treatment of solid tumors, such as breast cancer. In this work the effectiveness of CAR-T cells against monolayer and three-dimensional bioprinted tumor-like structures made of modified MCF-7 breast cancer cells was assessed. The cytokine profile of supernatants after co-cultivation of MCF-7 tumor cell models with CAR-T cells was also measured to reveal the inflammatory background associated with this interaction.

Keywords: Breast cancer; CAR-T; Immunotherapy; MCF-7; Solid tumor.