Study on the metabolic process of phthalic acid driven proliferation of Rhizoctonia solani

Jidong Ju; Bingqian Zhou; Guohong Yang; Xinyu Fu; Xiao Wang; Lanping Guo; Wei Liu

doi:10.3389/fpls.2023.1266916

Study on the metabolic process of phthalic acid driven proliferation of Rhizoctonia solani

Front Plant Sci. 2023 Oct 11:14:1266916. doi: 10.3389/fpls.2023.1266916. eCollection 2023.

Authors

Jidong Ju^{1

2

3}, Bingqian Zhou^{1

2}, Guohong Yang^{1

2}, Xinyu Fu^{1

2

3}, Xiao Wang^{1

2}, Lanping Guo⁴, Wei Liu^{1

2}

Affiliations

¹ Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
² Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
³ Pharmaceutical Institute, Shandong University of Traditional Chinese Medicine, Jinan, China.
⁴ Chinese Medicine Resource Center, China Academy of Chinese Medical Sciences, Beijing, China.

Abstract

Introduction: Continuous cropping obstacle seriously affects the quality and yield of Salvia miltiorrhiza, and the synergistic effect of root exudates and rhizosphere pathogenic microorganisms may be an important cause of continuous cropping obstacle. This study aimed to explore the effects of representative organic acids on the growth and metabolism of specific microorganisms in the S. miltiorrhiza rhizosphere soil under continuous cropping, and clarify its mechanism.

Methods: The effect of phthalic acid (PA) on the growth and metabolism of Rhizoctonia solani was evaluated by mycelial growth inhibition method. Ultra-high performance liquid chromatography and tandem mass spectrometry were used to identify the differential metabolites of R. solani induced by exogenous PA.

Results: PA exerted a concentration-dependent effect on mycelial growth, biomass, intracellular polysaccharides con-tent, and total protein content in R. solani. A total of 1773 metabolites and 1040 differential metabolites were identified in the blank medium (CK), Fungi (CK + fungi), and PA-Fungi (CK + fungi + acid) groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the differential metabolites were mainly involved in the sugar, lipid, and protein metabolic pathways related to stable membrane structure and cell growth.

Discussion: The proliferation and metabolism network of R. solani induced by PA was proposed, and the enhancement of sugar, lipid, and amino acid metabolism was presumed to be related to the active resistance of cells to organic acid stress. These results offer new in-sights into the effects of PA metabolism on promoting R. solani proliferation, and provide theoretical support for further optimizing the rhizosphere microecological environment of Salvia miltiorrhiza continuous cropping soil and reducing continuous cropping obstacle.

Keywords: Rhizoctonia solani; metabolism network; phthalic acid; synergistic damage; widely targeted metabolomics.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by National Natural Science Foundation of China (Grant No. 82173917), Major Innovation Projects in Shandong Province (Grant No. 2021ZDSYS12), National Modern Agricultural Industry Technology System (Grant No. CARS-21), Central-level Major Increase and Decrease Projects (Grant No.2060302), and Qilu University of Technology (Shandong Academy of Sciences) Science, Education and Industry Integration In-novation Pilot Project (Grant Nos. 2023PYI004 and 2022PX093).