Exopolysaccharide from Weissella confusa J4-1 inhibits colorectal cancer via induction of cell cycle arrest

Lei Liu; Yurong Du; Yabing Du; Weiliang Yan; Yuanzhe Li; Kang Cui; Zhen Li; Pu Yu; WanCun Zhang; Jianguo Feng; Wang Ma; Huan Zhao

doi:10.1016/j.ijbiomac.2023.127625

Exopolysaccharide from Weissella confusa J4-1 inhibits colorectal cancer via induction of cell cycle arrest

Int J Biol Macromol. 2023 Dec 31;253(Pt 8):127625. doi: 10.1016/j.ijbiomac.2023.127625. Epub 2023 Oct 24.

Authors

Lei Liu¹, Yurong Du¹, Yabing Du¹, Weiliang Yan¹, Yuanzhe Li², Kang Cui¹, Zhen Li³, Pu Yu¹, WanCun Zhang², Jianguo Feng⁴, Wang Ma⁵, Huan Zhao⁶

Affiliations

¹ Oncology department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
² Department of Pediatrics, Children's Hospital Affiliated of Zhengzhou University, Zhengzhou, China.
³ National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine, Heart Center of Henan Provincial People's Hospital, Central China Fuwai Hospital of Zhengzhou University, Fuwai Central China Cardiovascular Hospital & Central China Branch of National Center for Cardiovascular Diseases, Zhengzhou, Henan 451464, China.
⁴ Department of Anesthesiology, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China.
⁵ Oncology department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: doctormawang@126.com.
⁶ Oncology department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: zhaohuan912@126.com.

PMID: 37884233
DOI: 10.1016/j.ijbiomac.2023.127625

Abstract

Exopolysaccharide (EPS), a bioproduct of lactic acid bacteria (LAB), has various health-promoting biological activities that may be beneficial for cancer therapy. This in vivo and in vitro study aimed to elucidate the anti-colorectal cancer (CRC) capacity of a homopolysaccharide EPS obtained from Weissella confusa J4-1 (EPSJ4-1) isolated from the faeces of healthy infants. We confirmed that EPSJ4-1 contained glucose and effectively suppressed the proliferation, migration, and invasion of CRC cells. EPSJ4-1 treatment significantly retarded the growth of HT-29 tumour xenografts without causing cytotoxicity to normal organs. EPSJ4-1 exerts an inhibitory effect on cell proliferation by inducing G0/G1 phase cell cycle arrest in CRC cells. Furthermore, EPSJ4-1 upregulated p21 levels and downregulated mutant p53 and cyclin kinase 2 levels. This is the first study to demonstrate the antitumour effects of EPS from W. confusa on CRC via cell cycle arrest and inhibition of cell migration and invasion, suggesting that EPSJ4-1 has the potential to be developed as a nutraceutical or pharmaceutical drug to prevent and treat CRC.

Keywords: Colorectal cancer; Exopolysaccharide; W. confusa.

MeSH terms

Cell Cycle
Cell Cycle Checkpoints
Cell Proliferation
Colorectal Neoplasms* / metabolism
Humans
Infant
Weissella* / metabolism

Supplementary concepts

Weissella confusa