Surgical protocol for pulmonary artery banding in mice to generate a model of pressure-overload-induced right ventricular failure

Argen Mamazhakypov; Christine Veith; Ralph Theo Schermuly; Akylbek Sydykov

doi:10.1016/j.xpro.2023.102660

Surgical protocol for pulmonary artery banding in mice to generate a model of pressure-overload-induced right ventricular failure

STAR Protoc. 2023 Dec 15;4(4):102660. doi: 10.1016/j.xpro.2023.102660. Epub 2023 Oct 25.

Authors

Argen Mamazhakypov¹, Christine Veith¹, Ralph Theo Schermuly², Akylbek Sydykov³

Affiliations

¹ Department of Internal Medicine, Member of the German Center for Lung Research (DZL), Justus Liebig University of Giessen, Giessen, Germany.
² Department of Internal Medicine, Member of the German Center for Lung Research (DZL), Justus Liebig University of Giessen, Giessen, Germany. Electronic address: ralph.schermuly@innere.med.uni-giessen.de.
³ Department of Internal Medicine, Member of the German Center for Lung Research (DZL), Justus Liebig University of Giessen, Giessen, Germany. Electronic address: akylbek.sydykov@innere.med.uni-giessen.de.

Abstract

Right ventricular failure (RVF) is the leading cause of death in patients with pulmonary hypertension. Here, we present a protocol for pulmonary artery banding in mice to generate a model of pressure-overload-induced RVF. We describe steps for anesthesia of mice, endotracheal intubation, and pulmonary artery banding surgery. We then detail procedures for phenotyping and analysis. Our approach does not involve complete blockage of the pulmonary flow during clip placement and is, therefore, associated with low intraoperative mortality. For complete details on the use and execution of this protocol, please refer to Veith et al. (2020).¹.

Keywords: Cell Biology; Health Sciences; Model Organisms.

MeSH terms

Animals
Heart Failure* / etiology
Heart Failure* / surgery
Humans
Hypertension, Pulmonary* / etiology
Hypertension, Pulmonary* / surgery
Mice
Pulmonary Artery / surgery