Experimental investigations on PVA/chitosan and PVA/chitin films for active food packaging using Oxytenanthera abyssinica lignin nanoparticles and its UV-shielding, antimicrobial, and antiradical effects

Limenew Abate Worku; Mesfin Getachew Tadesse; Archana Bachheti; D P Pandey; Anuj Kumar Chandel; Asnake Waltenigus Ewuntu; Rakesh Kumar Bachheti

doi:10.1016/j.ijbiomac.2023.127644

Experimental investigations on PVA/chitosan and PVA/chitin films for active food packaging using Oxytenanthera abyssinica lignin nanoparticles and its UV-shielding, antimicrobial, and antiradical effects

Int J Biol Macromol. 2024 Jan;254(Pt 1):127644. doi: 10.1016/j.ijbiomac.2023.127644. Epub 2023 Oct 24.

Authors

Limenew Abate Worku¹, Mesfin Getachew Tadesse¹, Archana Bachheti², D P Pandey³, Anuj Kumar Chandel⁴, Asnake Waltenigus Ewuntu⁵, Rakesh Kumar Bachheti⁶

Affiliations

¹ Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa, P.O. Box 16417, Ethiopia; Bioprocess and Biotechnology Center of Excellence, Addis Ababa Sciences and Technology University, P.O. Box-16417, Addis Ababa, Ethiopia.
² Department of Environment Science, Graphic Era (Deemed to be University), Dehradun 248002, Uttarakhand, India. Electronic address: archanabachheti@geu.ac.in.
³ Department of Chemistry Govt Degree College Dehradun Shahar, Suddhowala, Dehradun 248007, India.
⁴ Department of Biotechnology, Engineering School of Lorena (EEL), Estrada Municipal do Campinho, University of São Paulo (USP), Lorena 12602-810, São Paulo, Brazil. Electronic address: anuj10@usp.br.
⁵ Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa, P.O. Box 16417, Ethiopia. Electronic address: asnake.waltenigus@aastu.edu.et.
⁶ Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa, P.O. Box 16417, Ethiopia; Department of Allied Sciences, Graphic Era Hill University, Society Area, Clement Town, Dehradun 248002, India. Electronic address: rkbachheti@gmail.com.

PMID: 37879578
DOI: 10.1016/j.ijbiomac.2023.127644

Abstract

This study investigated the effect of adding lignin nanoparticles (LNPs) derived from Oxytenanthera abyssinica via alkali-acid nanoprecipitation method to polyvinyl alcohol/chitosan (PVA/CI) and polyvinyl alcohol/chitin (PVA/CH) films for the active food packaging applications. Adding LNPs at concentrations of 1 % and 3 % improved the films' thermal stability and mechanical properties. The lowest water solubility and moisture content were observed in PVA/CI/LNPs films. LNPs exhibited effective 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, with the highest values observed in PVA/CH/LNP_S and PVA/CI/LNP_S films with values of 87.47 and 88.74 % respectively. The addition of LNPs also improved the UV-blocking abilities of the films. PVA/CH/LNP₃ and PVA/CI/LNP₃ have the smallest percentage transmission values of 3.34 % and 0.86 % in the UV range. The overall migration of dietary stimulants was lower in PVA/CI/LNP_S and PVA/CH/LNP_S films compared to PVA film. Antibacterial tests demonstrated the inhibitory capacity of the synthesized biofilms against both gram-positive and negative bacterial species, with the highest inhibitory value of 26 mm. The study suggests that PVA/CH/LNP_S and PVA/CI/LNP_S films have potential applications as active food packaging materials and can be explored in other potential applications such as drug delivery, tissue engineering, wound healing, and slow-release urea fertilizer development.

Keywords: Antibacterial activities; Antioxidant; Bio-nanocomposite films; Food packaging; Lignin nanoparticles; O. abyssinica; UV barrier.

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Anti-Infective Agents* / chemistry
Anti-Infective Agents* / pharmacology
Chitin
Chitosan* / chemistry
Food Packaging / methods
Lignin / chemistry
Lignin / pharmacology
Nanoparticles* / chemistry
Polyvinyl Alcohol / chemistry

Substances

Chitosan
Lignin
Chitin
Polyvinyl Alcohol
Anti-Infective Agents
Anti-Bacterial Agents