Zearalenone promotes porcine ESCs apoptosis by enhancing Drp1-mediated mitochondrial fragmentation and activating the JNK pathway

Sirao Hai; Jie Zhao; Chuangjiang Chen; Chenlong Wang; Li Ma; Sajid Ur Rahman; Chang Zhao; Shibin Feng; Jinjie Wu; Xichun Wang

doi:10.1016/j.fct.2023.114110

Zearalenone promotes porcine ESCs apoptosis by enhancing Drp1-mediated mitochondrial fragmentation and activating the JNK pathway

Food Chem Toxicol. 2023 Dec:182:114110. doi: 10.1016/j.fct.2023.114110. Epub 2023 Oct 23.

Authors

Affiliations

¹ College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, China.
² College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, China; Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
³ College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, China; Anhui Province Engineering Laboratory for Animal Food Quality and Bio-safety, Hefei, 230036, China. Electronic address: wjj@ahau.edu.cn.
⁴ College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, China; Anhui Province Engineering Laboratory for Animal Food Quality and Bio-safety, Hefei, 230036, China. Electronic address: wangxichun@ahau.edu.cn.

PMID: 37879531
DOI: 10.1016/j.fct.2023.114110

Abstract

Zearalenone (ZEA) is widely present in food and feed, and pigs are susceptible to its effects. This study explored the underlying function of ZEA-induced apoptosis in porcine endometrial stromal cells (ESCs) through activation of the JNK signaling pathway and mitochondrial division. This study utilized ESCs to explore the impact of exposure to ZEA. A mitochondrial division inhibitor (Mdivi) was also included as a reference. The results indicated a gradual decrease in cell viability with increasing ZEA concentration. In addition, ZEA can modify the growth status of porcine ESCs, disrupt their ultrastructure, and lead to apoptosis of porcine ESCs via the mitochondrial division pathway and JNK signaling pathway. In summary, our study found the critical targets of ZEA infected with pig ESCs, which provided a conceptual foundation to prevent and control ZEA.

Keywords: Drp 1; JNK signaling pathway; Mitochondrial fission; Porcine endometrial stromal cells; Zearalenone.

MeSH terms

Animals
Apoptosis
MAP Kinase Signaling System / physiology
Stromal Cells
Swine
Zearalenone* / metabolism
Zearalenone* / toxicity

Substances

Zearalenone