Sewage surveillance provides useful epidemiologic and public health information on viral infections at the population level. We detected monkeypox virus DNA from sewage samples covering 85% of the population in Santiago Metropolitan Region Chile. We also isolated infective viruses from those samples. Wastewater surveillance could complement clinical surveillance for monkeypox virus.
Keywords: Chile; MPXV; Santiago; infectivity; metropolitan region; monkeypox virus; mpox; sewage; viruses; wastewater samples; wastewater-based epidemiology; zoonoses.