Analysis of the apoplast fluid proteome during the induction of systemic acquired resistance in Arabidopsis thaliana

Shuna Jiang; Liying Pan; Qingfeng Zhou; Wenjie Xu; Fuge He; Lei Zhang; Hang Gao

doi:10.7717/peerj.16324

Analysis of the apoplast fluid proteome during the induction of systemic acquired resistance in Arabidopsis thaliana

PeerJ. 2023 Oct 20:11:e16324. doi: 10.7717/peerj.16324. eCollection 2023.

Authors

Shuna Jiang¹, Liying Pan², Qingfeng Zhou², Wenjie Xu², Fuge He², Lei Zhang³, Hang Gao²

Affiliations

¹ College of Survey and Planning, Shangqiu Normal University, Shangqiu, China.
² College of Biology and Food, Shangqiu Normal University, Shangqiu, China.
³ Institute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, China.

Abstract

Background: Plant-pathogen interactions occur in the apoplast comprising the cell wall matrix and the fluid in the extracellular space outside the plasma membrane. However, little is known regarding the contribution of the apoplastic proteome to systemic acquired resistance (SAR).

Methods: Specifically, SAR was induced by inoculating plants with Pst DC3000 avrRps4. The apoplast washing fluid (AWF) was collected from the systemic leaves of the SAR-induced or mock-treated plants. A label free quantitative proteomic analysis was performed to identified the proteins related to SAR in AWF.

Results: A total of 117 proteins were designated as differentially accumulated proteins (DAPs), including numerous pathogenesis-related proteins, kinases, glycosyl hydrolases, and redox-related proteins. Functional enrichment analyses shown that these DAPs were mainly enriched in carbohydrate metabolic process, cell wall organization, hydrogen peroxide catabolic process, and positive regulation of catalytic activity. Comparative analysis of proteome data indicated that these DAPs were selectively enriched in the apoplast during the induction of SAR.

Conclusions: The findings of this study indicate the apoplastic proteome is involved in SAR. The data presented herein may be useful for future investigations on the molecular mechanism mediating the establishment of SAR.

Keywords: Apoplast; Differentially accumulated proteins; Extracellular space; Proteomics; Systemic acquired resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis Proteins* / genetics
Arabidopsis* / metabolism
Cell Membrane / metabolism
Proteome / metabolism
Proteomics

Substances

Proteome
Arabidopsis Proteins

Grants and funding

This work was supported by the Key Scientific Research Project of Colleges and Universities in Henan Province (No. 23B210004), and the Natural Science Foundation of Henan Province (222300420259). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.