Functional characterization of alkaline phosphatases involved alarm pheromone in the vetch aphid Megoura viciae

Xuan Song; Yao-Guo Qin; Yi-Han Zhang; Yu-Bei Zhou; Dan Chen; Dong-Hai Xie; Zheng-Xi Li

doi:10.1016/j.isci.2023.108115

Functional characterization of alkaline phosphatases involved alarm pheromone in the vetch aphid Megoura viciae

iScience. 2023 Oct 1;26(11):108115. doi: 10.1016/j.isci.2023.108115. eCollection 2023 Nov 17.

Authors

Xuan Song¹, Yao-Guo Qin¹, Yi-Han Zhang¹, Yu-Bei Zhou¹, Dan Chen¹, Dong-Hai Xie¹, Zheng-Xi Li¹

Affiliation

¹ Department of Entomology and MOA Key Laboratory for Monitoring and Environment-Friendly Control of Crop Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China.

Abstract

The alkaline phosphatases (ALPs) are highly promiscuous enzymes and have been extensively investigated in mammals for their medical significance, but their functional promiscuity is relatively poorly understood in insects. Here, we first identified four ALP genes (designated as MvALP1-4) in the vetch aphid Megoura viciae that contained one alkaline phosphatase site, three metal-binding sites, and varied other functional sites. Phylogenetic analysis, molecular docking and the spatiotemporal expression profiling of MvALP1-4 were very different, indicating a promiscuous functionality. We also found that MvALP4 involved the biosynthesis of aphid alarm pheromones (EβF) in vitro and in vivo. Finally, transcriptome analysis in the stimulated and unstimulated aphids supported the involvement of MvALPs in the biosynthesis of aphid alarm pheromones. Our study identified a multifunctional ALP involved terpene synthase enzyme activity in the aphid, which contributes to the understanding of the functional plasticity of ALPs in insects.

Keywords: Entomology; Molecular biology; Phylogeny.