Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles

Juan Chen; Yuhuan Lv; Weilong Shang; Yi Yang; Yuting Wang; Zhen Hu; Xiaonan Huang; Rong Zhang; Jizhen Yuan; Jingbin Huang; Xiancai Rao

doi:10.3389/fmicb.2023.1254367

Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles

Front Microbiol. 2023 Oct 6:14:1254367. doi: 10.3389/fmicb.2023.1254367. eCollection 2023.

Authors

Juan Chen^#¹, Yuhuan Lv^#^{2

3}, Weilong Shang^#², Yi Yang², Yuting Wang², Zhen Hu², Xiaonan Huang², Rong Zhang¹, Jizhen Yuan³, Jingbin Huang¹, Xiancai Rao²

Affiliations

¹ Department of Pharmacy, The Second Affiliated Hospital, Army Medical University, Chongqing, China.
² Department of Microbiology, College of Basic Medical Science, Army Medical University, Chongqing, China.
³ Department of Clinical Laboratory, The 971st Hospital of Chinese People's Liberation Army Navy, Qingdao, China.

^# Contributed equally.

Abstract

Background: Membrane vesicles (MVs) are nanoscale vesicular structures produced by bacteria during their growth in vitro and in vivo. Some bacterial components can be loaded in bacterial MVs, but the roles of the loaded MV molecules are unclear.

Methods: MVs of Staphylococcus aureus RN4220 and its derivatives were prepared. Dynamic light scattering analysis was used to evaluate the size distribution, and 4D-label-free liquid chromatography-tandem mass spectrometry analysis was performed to detect protein composition in the MVs. The site-mutation S. aureus RN4220-Δhld and agrA deletion mutant RN4220-ΔagrA were generated via allelic replacement strategies. A hemolysis assay was performed with rabbit red blood cells. CCK-8 and lactate dehydrogenase release assays were used to determine the cytotoxicity of S. aureus MVs against RAW264.7 macrophages. The serum levels of inflammatory factors such as IL-6, IL-1β, and TNFα in mice treated with S. aureus MVs were detected with an enzyme-linked immunosorbent assay kit.

Results: Delta-hemolysin (Hld) was identified as a major loaded factor in S. aureus MVs. Further study showed that Hld could promote the production of staphylococcal MVs with smaller sizes. Loaded Hld affected the diversity of loaded proteins in MVs of S. aureus RN4220. Hld resulted in decreased protein diversity in MVs of S. aureus. Site-mutation (RN4220-Δhld) and agrA deletion (RN4220-ΔagrA) mutants produced MVs (^ΔhldMVs and ^ΔagrAMVs) with a greater number of bacterial proteins than those derived from wild-type RN4220 (^wtMVs). Moreover, Hld contributed to the hemolytic activity of ^wtMVs. Hld-loaded ^wtMVs were cytotoxic to macrophage RAW264.7 cells and could stimulate the production of inflammatory factor IL-6 in vivo.

Conclusion: This study presented that Hld was a major loaded factor in S. aureus MVs, and the loaded Hld played vital roles in the MV-property modification.

Keywords: Hld; Staphylococcus aureus; agrA; inflammatory factor; membrane vesicle.

Grants and funding

This study was supported by the National Natural Science Foundation of China (grant numbers 82302063 to JC and 32000652 to JY).