Identification of Puccinia striiformis races from the spring wheat crop in Xinjiang, China

Jinbiao Ma; Muhammad Awais; Li Chen; Hong Yang; Hanlin Lai; Yuyang Shen; Huiqing Wang; Guangkuo Li; Haifeng Gao

doi:10.3389/fpls.2023.1273306

Identification of Puccinia striiformis races from the spring wheat crop in Xinjiang, China

Front Plant Sci. 2023 Oct 3:14:1273306. doi: 10.3389/fpls.2023.1273306. eCollection 2023.

Authors

Jinbiao Ma¹, Muhammad Awais², Li Chen³, Hong Yang⁴, Hanlin Lai⁵, Yuyang Shen³, Huiqing Wang⁶, Guangkuo Li³, Haifeng Gao³

Affiliations

¹ State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.
² State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Shaanxi, Xianyang, China.
³ Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, Urumqi, China.
⁴ College of Agriculture, Xinjiang Agricultural University/Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests, Urumqi, China.
⁵ College of Life Science, Xinjiang Agricultural University, Urumqi, China.
⁶ Xinjiang Plant Protection Station, Department of Xinjiang Agriculture, Urumqi, China.

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a foliar disease that affects both winter and spring wheat crops in Xinjiang, China, which is linked to Central Asia. Race identification of Pst from spring wheat in Xinjiang was not done before. In this study, a total of 216 isolates were recovered from stripe rust samples of spring wheat in the region in 2021 and multiplied using the susceptible cultivar Mingxian 169. These isolates were tested on the Chinese set of 19 wheat differential lines for identifying Pst races. A total of 46 races were identified. Races Suwon-11-1, Suwon11-12, and CYR32 had high frequencies in the spring wheat region. The frequencies of virulence factors on differentials "Fulhard" and "Early Premium" were high (>95%), whereas the virulence factor to differential "Triticum spelta var. Album" (Yr5) was not detected, while virulence to other differentials showed variable frequency within different counties. The predominant races in winter wheat in the same season were also detected from spring wheat cultivars, indicating Pst spreading from winter wheat to spring wheat crops. Deploying resistance genes in spring and winter wheat cultivars is critical for control stripe rust.

Keywords: China epidemic regions; race identification; spring wheat; virulence factor; wheat stripe rust.

Grants and funding

The authors declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by the Natural Science Foundation of Xinjiang Uygur Autonomous Region (2022D01A270), the Xinjiang Uygur Autonomous Region, Regional Coordinated Innovation Project (Shanghai Cooperation Organization Science and Technology Partnership Program) (No. 2022E01022), the Science and Technology Assistance Project of Xinjiang Uygur Autonomous Region (2022E02070), the Key Research & Development Program of Xinjiang (2022B02015-3 and 2021B02002-1), the Urumqi Integrated Experimental Station of China Agriculture Research System for Wheat (CARS-03-88), the Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis (KFJJ202104), and the Project of Fund for Stable Support to Agricultural Sci-Tech Renovation (xjnkywdzc-2022004).