Toxicity assessment of perfluorooctanesulfonic acid (PFOS) on a spontaneous plant, velvetleaf (Abutilon theophrasti), via metabolomics

Abstract

Spontaneous plants often play important ecological roles in terrestrial environments, but impacts of contaminants on spontaneous plants are seldom investigated. Per- and polyfluoroalkyl substances (PFAS), such as perfluorooctanesulfonic acid (PFOS) are ubiquitous in rural and urban soils. In this study, we assessed the effects of PFOS on a spontaneous plant, velvetleaf (Abutilon theophrasti), using endpoints such as plant growth, stress defense, PFOS uptake, and elemental and metabolite profile. We observed stunted growth in plants grown in PFOS-contaminated soils, with PFOS accumulating in their shoots by up to 3000 times more than the control plants. The other endpoints (decreased chlorophyll a synthesis, elevated oxidative stress, reduced shoot Mg concentration, and reduced biomass production) also explained the stunted growth of velvetleaf exposed to elevated PFOS concentrations. We found that 56 metabolites involved in 13 metabolic pathways were dysregulated. The synthesis of important antioxidants such as ascorbic acid, hydroxycinnamic acids (coumaric, caffeic, ferulic, and sinapic acids), and tocopherols decreased, resulting in loss of plant's defense to stress. PFOS also reduced the levels of growth-related and stress-coping metabolites including squalene, serotonin, noradrenalin, putrescine, and indole-3-propionic acid, which further corroborated the restricted growth of velvetleaf exposed to elevated PFOS. These findings provide insights on phytotoxicity of PFOS to velvetleaf, a resilient terrestrial spontaneous plant.

Keywords: PFAS; Soil contamination; Stress regulation; Urban soil; Weed; Wild plants.