Gold nanoparticles (AuNPs) have emerged as powerful tools in the construction of highly sensitive electrochemical biosensors. Their unique properties, such as the ability to serve as an effective platform for biomolecule immobilization and to facilitate electron transfer between the electrode surface and the immobilized molecules, make them a promising choice for biosensor applications. Utilizing AuNPs modified electrodes can lead to improved sensitivity and lower limits of detection compared to unmodified electrodes. This review provides a comprehensive overview of the recent advancements and applications of AuNPs-based electrochemical biosensors in the biomedical field. The synthesis methods of AuNPs, their key properties, and various strategies employed for electrode modification are discussed. Furthermore, this review highlights the remarkable applications of these nanostructure-integrated electrodes, including immunosensors, enzyme biosensors, and DNA biosensors.
Keywords: DNA biosensors; Enzyme biosensors; Gold nanoparticles (AuNPs); Immunosensors; Nanostructured electrodes; Point-of-care testing (POCT).
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