Chitosan modified graphene field-effect transistor biosensor for ultrasensitive procalcitonin detection

Furong Chen; Ying Zhang; Mingxuan Wang; Jinghai Liu; Wenfeng Hai; Yushuang Liu

doi:10.1016/j.talanta.2023.125308

Chitosan modified graphene field-effect transistor biosensor for ultrasensitive procalcitonin detection

Talanta. 2024 Feb 1;268(Pt 1):125308. doi: 10.1016/j.talanta.2023.125308. Epub 2023 Oct 13.

Authors

Furong Chen¹, Ying Zhang¹, Mingxuan Wang¹, Jinghai Liu¹, Wenfeng Hai², Yushuang Liu³

Affiliations

¹ Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), College Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao, 028000, People's Republic of China.
² Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), College Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao, 028000, People's Republic of China. Electronic address: hai_wenfeng@163.com.
³ Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), College Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao, 028000, People's Republic of China; Key Laboratory of Mongolian Medicine Research and Development Engineering, Ministry of Education, Inner Mongolia Minzu University, Tongliao, 028000, People's Republic of China. Electronic address: yushuangliu1989@163.com.

PMID: 37862752
DOI: 10.1016/j.talanta.2023.125308

Abstract

Sepsis is a systemic inflammatory response caused by a bacterial infection that often leading to tissue damage, organ failure and death. Procalcitonin (PCT), as a peptide precursor to hormones, is the main biomarker to identification of the sepsis. In this study, a chitosan modified graphene field transistor (CTS-GFET) was established and first time used for PCT ultra-sensitive detection. CTS was functionalized on the GFET channel surface to immobilized anti-PCT by glutaraldehyde. This biosensor exhibited the detection limit as low as 0.82 ag/mL in PBS, which exhibited 3 times enhancement compared with GFET biosensors. The enhancement mechanisms of CTS-GFET were studied by electrical theoretical model. In addition, the CTS-GFET biosensor was successfully applied to quantify the concentration of the PCT in human serum samples, indicating the potential use in clinical application.

Keywords: Chitosan; Graphene field-effect transistor; Procalcitonin; Sepsis.

MeSH terms

Biosensing Techniques*
Chitosan*
Graphite*
Humans
Procalcitonin
Sepsis*

Substances

Procalcitonin
Graphite
Chitosan