Nanoparticles fabricated from the bioactive tilapia scale collagen for wound healing: Experimental approach

Manal Shalaby; Dalia Hamouda; Shaimaa M Khedr; Haitham M Mostafa; Hesham Saeed; Ahmed Z Ghareeb

doi:10.1371/journal.pone.0282557

Nanoparticles fabricated from the bioactive tilapia scale collagen for wound healing: Experimental approach

PLoS One. 2023 Oct 20;18(10):e0282557. doi: 10.1371/journal.pone.0282557. eCollection 2023.

Authors

Manal Shalaby^{1

2}, Dalia Hamouda¹, Shaimaa M Khedr², Haitham M Mostafa¹, Hesham Saeed³, Ahmed Z Ghareeb²

Affiliations

¹ Medical Biotechnology Department, Institute of Genetic Engineering and Biotechnology, City of Scientific Research and Technological Applications, Alexandria, Egypt.
² Centre of Excellence for Drug Preclinical Studies (CE-DPS), Pharmaceutical and Fermentation Industry Development Centre, City of Scientific Research and Technological Applications, New Borg El Arab, Alexandria, Egypt.
³ Department of Biotechnology, Institute of Graduate Studies and Research (IGSR), Alexandria University, Alexandria, Egypt.

Abstract

The creation of innovative wound-healing nanomaterials based on natural compounds emerges as a top research goal. This research aimed to create a gel containing collagen nanoparticles and evaluate its therapeutic potential for skin lesions. Collagen nanoparticles were produced from fish scales using desolvation techniques. Using SDS PAGE electrophoresis, Fourier transform infrared spectroscopy (FTIR) as well as the structure of the isolated collagen and its similarities to collagen type 1 were identified. The surface morphology of the isolated collagen and its reformulation into nanoparticles were examined using transmission and scanning electron microscopy. A Zeta sizer was used to examine the size, zeta potential, and distribution of the synthesized collagen nanoparticles. The cytotoxicity of the nanomaterials was investigated and an experimental model was used to evaluate the wound healing capability. The overall collagen output from Tilapia fish scales was 42%. Electrophoretic patterns revealed that the isolated collagen included a unique protein with chain bands of 126-132 kDa and an elevated beta band of 255 kDa. When compared to the isolated collagen, the collagen nanoparticles' FTIR results revealed a significant drop in the amide II (42% decrease) and amide III (32% decrease) band intensities. According to SEM analysis, the generated collagen nanoparticles ranged in size from 100 to 350 nm, with an average diameter of 182 nm determined by the zeta sizer. The produced collagen nanoparticles were polydispersed in nature and had an equivalent average zeta potential of -17.7 mV. Cytotoxicity study showed that, when treating fibroblast cells with collagen nanoparticle concentrations, very mild morphological alterations were detected after human skin fibroblasts were treated with collagen nanoparticles 32 μg/ml for 24 hours, as higher concentrations of collagen nanoparticles caused cell detachment. Macroscopical and histological investigations proved that the fabricated fish scale collagen nanoparticles promoted the healing process in comparison to the saline group.

Copyright: © 2023 Shalaby et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amides
Animals
Collagen / metabolism
Humans
Nanoparticles*
Tilapia* / metabolism
Wound Healing

Substances

Collagen
Amides

Grants and funding

This research was funded by the Academy of Scientific Research and Technology (ASRT), Grant number 26D/2017. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.