Anti-inflammatory activities of Levilactobacillus brevis KU15147 in RAW 264.7 cells stimulated with lipopolysaccharide on attenuating NF-κB, AP-1, and MAPK signaling pathways

Jun-Hyun Hyun; Hyung-Seok Yu; Im-Kyung Woo; Gil-Woong Lee; Na-Kyoung Lee; Hyun-Dong Paik

doi:10.1007/s10068-023-01318-w

Anti-inflammatory activities of Levilactobacillus brevis KU15147 in RAW 264.7 cells stimulated with lipopolysaccharide on attenuating NF-κB, AP-1, and MAPK signaling pathways

Food Sci Biotechnol. 2023 Apr 26;32(14):2105-2115. doi: 10.1007/s10068-023-01318-w. eCollection 2023 Dec.

Authors

Jun-Hyun Hyun¹, Hyung-Seok Yu¹, Im-Kyung Woo¹, Gil-Woong Lee², Na-Kyoung Lee¹, Hyun-Dong Paik¹

Affiliations

¹ Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029 Republic of Korea.
² View of Creativity, GHBio Co., Ltd., 120 Neungdong-Ro, Seoul, Republic of Korea.

Abstract

Probiotics confer many beneficial effects on several illnesses, ranging from microbial diarrhea to inflammatory diseases. This study was conducted on whether Levilactobacillus brevis KU15147 obtained from kimchi has anti-inflammatory effects in RAW 264.7 cells stimulated with lipopolysaccharide (LPS) and antioxidant potential. L. brevis KU15147 reduced nitric oxide and prostaglandin E₂ levels with decreasing the activation of inducible nitric oxide synthase and cyclooxygenase-2 without cell cytotoxicity. In addition, L. brevis KU15147 attenuated proinflammatory cytokine production including tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 in RAW 264.7 cells stimulated with LPS. Additionally, L. brevis KU15147 reduced the activity of nuclear factor-κB, activator protein-1, and mitogen-activated protein kinase signaling pathways. Furthermore, L. brevis KU15147 downregulated the production of reactive oxygen species. Therefore, L. brevis KU15147 was concluded that had an inhibition effect on LPS-induced inflammatory responses and can be used in functional foods to suppress inflammatory diseases.

Supplementary information: The online version contains supplementary material available at 10.1007/s10068-023-01318-w.

Keywords: AP-1 signaling; Anti-inflammatory; Levilactobacillus brevis; MAPK signaling; NF-κB signaling.

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