Study on extraction technology and antioxidant activity of total alkaloids from Hemsleya chinensis based on orthogonal design and BP neural network

Weiwei Jiang; Shaoyu Zheng; Chengxiao Yuan; Qingqing Gao; Chunfan Xiang; Shunwei Tian; Jianmei Li; Yan Zhao

doi:10.1016/j.heliyon.2023.e20680

Study on extraction technology and antioxidant activity of total alkaloids from Hemsleya chinensis based on orthogonal design and BP neural network

Heliyon. 2023 Oct 9;9(10):e20680. doi: 10.1016/j.heliyon.2023.e20680. eCollection 2023 Oct.

Authors

Weiwei Jiang^{1

2}, Shaoyu Zheng¹, Chengxiao Yuan^{1

3}, Qingqing Gao^{1

3}, Chunfan Xiang^{1

3}, Shunwei Tian¹, Jianmei Li¹, Yan Zhao^{1

2

3}

Affiliations

¹ National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
² Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, 650106, China.
³ College of Agronomy and Biotechnology, Yunnan Agricultural University, Yunnan, 650201, China.

Abstract

In this study, total alkaloids from Hemsleya chinensis were extracted and tested for their antioxidant properties. To optimize extraction methods, a single factor experiment was conducted to determine the total alkaloid concentrations of H. chinensis using the L₉ (3⁴) orthogonal design test method and the BP neural network (BPNN), resulting in the optimum extraction conditions for total alkaloids. The optimal conditions for H. chinensis alkaloids extraction with acid water are: HCl concentration is 0.50 %, extraction temperature is 85 °C, material-liquid ratio is 1:64.5, and extraction rate of alkaloids is 0.2785 ± 0.0003 mg/mL. The alkaloid from H. chinensis exhibited antioxidant activity in a quantity-effect relationship with activity. These findings showed that the procedure to be reasonable, the alkaloid extraction efficiency to be high, and the method could be used to extract the alkaloids of H. chinensis, improving the development of natural and healthy medicinal resources for the pharmaceutical and food industries.

Keywords: Alkaloids; Antioxidant activity; BPNN; Hemsleya chinensis; Orthogonal design.