Biocompatible and antibacterial poly(lactic acid)/cellulose nanofiber‑silver nanoparticle biocomposites prepared via Pickering emulsion method

Chun-Nan Wu; Te-En Wang; Chen-Che Hsieh; Kuan-Chen Cheng; Kevin C-W Wu

doi:10.1016/j.ijbiomac.2023.127495

Biocompatible and antibacterial poly(lactic acid)/cellulose nanofiber‑silver nanoparticle biocomposites prepared via Pickering emulsion method

Int J Biol Macromol. 2023 Dec 31;253(Pt 8):127495. doi: 10.1016/j.ijbiomac.2023.127495. Epub 2023 Oct 17.

Authors

Chun-Nan Wu¹, Te-En Wang², Chen-Che Hsieh³, Kuan-Chen Cheng⁴, Kevin C-W Wu⁵

Affiliations

¹ Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 10617, Taiwan. Electronic address: wulalaworld@gmail.com.
² Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 10617, Taiwan.
³ Institute of Biotechnology, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 10617, Taiwan.
⁴ Institute of Biotechnology, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 10617, Taiwan; Institute of Food Science Technology, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 10617, Taiwan; Department of Optometry, Asia University, No. 500, Lioufeng Rd., Taichung 413305, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, No. 91, Hsueh-Shih Road, Taichung 404333, Taiwan.
⁵ Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 10617, Taiwan; Institute of Biomedical Engineering & Nanomedicine, National Health Research Institute, No. 35, Keyan Road, Zhunan Town, Miaoli County 35053, Taiwan. Electronic address: kevinwu@ntu.edu.tw.

PMID: 37858642
DOI: 10.1016/j.ijbiomac.2023.127495

Abstract

Developing biocompatible and antibacterial materials with biodegradable polymers is an ideal strategy to improve public health problems and plastic pollution simultaneously. In the present study, novel biocompatible and antibacterial poly (_L-lactic acid) (PLLA, coded as P)/TEMPO-oxidized cellulose nanofiber (TOCNF, coded as T)‑silver nanoparticle (AgNP, coded as A) films were first developed. The core/shell PT Pickering emulsion was prepared by sonication treatment. The TOCNF shells with -COO^-Na⁺ groups (∼1.5 mmol/g cellulose) were used as the support to in situ synthesize and immobilize AgNPs on the PT emulsion droplets. Silver nitrate (AgNO₃) (1.5, 3.0, 4.5, and 6.0 mmol/g cellulose) was added to the PT emulsions. Then, ion-exchange reaction and hydrothermal reduction were conducted to form PTA (PTA1-PTA4) emulsions. After centrifugation to remove the excess Ag⁺, filtration, oven-drying, and hot-pressing, the PTA composite films were successfully prepared. The PTA3 film contained AgNPs 12.4 ± 2.8 nm in diameter and exhibited the highest antibacterial activities against the E. coli (85.2%) and S. aureus (80.1%) at 37 °C, where the initial bacterial suspension concentrations were approximately 2 × 10⁸ CFU mL^-1. Therefore, the biocompatible and antibacterial PTA3 film is a promising candidate for biomedical applications, in particular as an antibacterial bioactive packaging material.

Keywords: Antibacterial activity; Biocompatibility; Cellulose nanofiber; PLA; Silver nanoparticle; TEMPO.

MeSH terms

Anti-Bacterial Agents / pharmacology
Cellulose
Emulsions
Escherichia coli
Metal Nanoparticles*
Nanofibers*
Silver
Staphylococcus aureus

Substances

Cellulose
poly(lactide)
Emulsions
Silver
Anti-Bacterial Agents