Development of fluorescent probes with specific recognition moiety for hydrogen polysulfide

Xia Zhang; Yang Yang; Li Zhang; Shudi Liu; Zhihua Song; Liangwei Zhang; Jinmao You; Lingxin Chen

doi:10.1016/j.talanta.2023.125293

Development of fluorescent probes with specific recognition moiety for hydrogen polysulfide

Talanta. 2024 Feb 1;268(Pt 1):125293. doi: 10.1016/j.talanta.2023.125293. Epub 2023 Oct 12.

Authors

Xia Zhang¹, Yang Yang², Li Zhang³, Shudi Liu⁴, Zhihua Song⁵, Liangwei Zhang⁶, Jinmao You⁷, Lingxin Chen⁸

Affiliations

¹ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China; Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, 264003, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
² CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
³ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
⁴ College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005, China.
⁵ School of Pharmacy, Yantai University, Yantai, 264005, China.
⁶ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China. Electronic address: liangweizhang@yic.ac.cn.
⁷ College of Chemistry and Chemical Engineering, Shaoxing University, Shaoxing, 312000, China. Electronic address: jmyou6304@163.com.
⁸ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China. Electronic address: lxchen@yic.ac.cn.

PMID: 37857112
DOI: 10.1016/j.talanta.2023.125293

Abstract

Hydrogen polysulfide (H₂S_n, n > 1) is an important component of reactive sulfur species (RSS), which is an important substance for maintaining the redox balance in cells. However, limited recognition moieties are available for hydrogen polysulfide probe design. In this study, we have constructed a small library containing several organic molecules to explore a new specific recognition moiety for H₂S_n fluorescent probe design. To validate the discovery, two fluorescent probes, 7 and BCC, were further developed based on coumarin and its derivative. The probes exhibited desirable specificity for H₂S_n monitoring, which can be used for detecting H₂S_n in solution and cells. The new specific recognition moiety for H₂S_n fluorescent probe design discovered in this work has certain guiding significance for development of H₂S_n probes exploring biological roles in the future.

Keywords: Fluorescent probe; H(2)S(n); Living cells imaging; New recognition moiety; Probe design.

MeSH terms

Fluorescent Dyes*
Hydrogen
Hydrogen Sulfide*
Sulfides

Substances

polysulfide
Fluorescent Dyes
Hydrogen
Sulfides
Hydrogen Sulfide