Feed-forward metabotropic signaling by Cav1 Ca2+ channels supports pacemaking in pedunculopontine cholinergic neurons

Abstract

Like a handful of other neuronal types in the brain, cholinergic neurons (CNs) in the pedunculopontine nucleus (PPN) are lost during Parkinson's disease (PD). Why this is the case is unknown. One neuronal trait implicated in PD selective neuronal vulnerability is the engagement of feed-forward stimulation of mitochondrial oxidative phosphorylation (OXPHOS) to meet high bioenergetic demand, leading to sustained oxidant stress and ultimately degeneration. The extent to which this trait is shared by PPN CNs is unresolved. To address this question, a combination of molecular and physiological approaches were used. These studies revealed that PPN CNs are autonomous pacemakers with modest spike-associated cytosolic Ca²⁺ transients. These Ca²⁺ transients were partly attributable to the opening of high-threshold Ca_v1.2 Ca²⁺ channels, but not Ca_v1.3 channels. Ca_v1.2 channel signaling through endoplasmic reticulum ryanodine receptors stimulated mitochondrial OXPHOS to help maintain cytosolic adenosine triphosphate (ATP) levels necessary for pacemaking. Inhibition of Ca_v1.2 channels led to the recruitment of ATP-sensitive K⁺ channels and the slowing of pacemaking. A 'side-effect' of Ca_v1.2 channel-mediated stimulation of mitochondria was increased oxidant stress. Thus, PPN CNs have a distinctive physiological phenotype that shares some, but not all, of the features of other neurons that are selectively vulnerable in PD.

Keywords: ATP-sensitive K(+) channels; Calcium voltage-dependent channels; Mitocondrial oxidative phosphorilation; PPN cholinergic neurons; Parkinson's disease.