A novel label-free electrochemical immunosensor for the detection of heat shock protein 70 of lung adenocarcinoma cell line following paclitaxel treatment using l-cysteine-functionalized Au@MnO2/MoO3 nanocomposites

Yi-An Chen; Ming-You Shie; Chia-Che Ho; Sheng-Wen Ye; I-Wen Peter Chen; Yu-Yin Shih; Yu-Fang Shen; Yi-Wen Chen

doi:10.1039/d3ra03620k

A novel label-free electrochemical immunosensor for the detection of heat shock protein 70 of lung adenocarcinoma cell line following paclitaxel treatment using l-cysteine-functionalized Au@MnO₂/MoO₃ nanocomposites

RSC Adv. 2023 Oct 12;13(43):29847-29861. doi: 10.1039/d3ra03620k. eCollection 2023 Oct 11.

Authors

Yi-An Chen¹, Ming-You Shie^{1

2

3}, Chia-Che Ho⁴, Sheng-Wen Ye², I-Wen Peter Chen⁵, Yu-Yin Shih¹, Yu-Fang Shen⁴, Yi-Wen Chen^{1

2

4

6}

Affiliations

¹ x-Dimension Center for Medical Research and Translation, China Medical University Hospital Taichung City 404332 Taiwan.
² The Master Program for Biomedical Engineering, China Medical University Taichung City 406040 Taiwan.
³ Department of Biomedical Engineering, China Medical University Taichung City 40447 Taiwan.
⁴ Department of Bioinformatics and Medical Engineering, Asia University Taichung City 41354 Taiwan.
⁵ Department of Chemistry, National Cheng Kung University Tainan 70101 Taiwan.
⁶ Graduate Institute of Biomedical Sciences, China Medical University Taichung City 40447 Taiwan.

Abstract

The future trend in achieving precision medicine involves the development of non-invasive cancer biomarker sensors that offer high accuracy, low cost, and time-saving benefits for risk clarification, early detection, disease detection, and therapeutic monitoring. A facile approach for the synthesis of MoO₃ nanosheets was developed by thermally oxidizing MoS₂ nanosheets in air followed by thermal annealing. Subsequently, Au@MnO₂ nanocomposites were prepared using a combined hydrothermal process and in situ chemical synthesis. In this study, we present a novel immunosensor design strategy involving the immobilization of antiHSP70 antibodies on Au@MnO₂/MoO₃ nanocomposites modified on a screen-printed electrode (SPE) using EDC/NHS chemistry. This study establishes HSP70 as a potential biomarker for monitoring therapeutic response during anticancer therapy. Impedance measurements of HSP70 on the Au@MnO₂/MoO₃/SPE immunosensor using EIS showed an increase in impedance with an increase in HSP70 concentration. The electrochemical immunosensor demonstrated a good linear response in the range of 0.001 to 1000 ng mL^-1 with a detection limit of 0.17 pg mL^-1 under optimal conditions. Moreover, the immunosensor was effective in detecting HSP70 at low concentrations in a lung adenocarcinoma cell line following Paclitaxel treatment, indicating its potential for early detection of the HSP70 biomarker in organ-on-a-chip and clinical applications.