Preparation of N-Linked-Type GlcNAc Monomers for Glycopolymers and Binding Specificity for Lectin

Takahiko Matsushita; Momoka Nozaki; Mio Sunaga; Tetsuo Koyama; Ken Hatano; Koji Matsuoka

doi:10.1021/acsomega.3c05151

Preparation of N-Linked-Type GlcNAc Monomers for Glycopolymers and Binding Specificity for Lectin

ACS Omega. 2023 Sep 26;8(40):37329-37340. doi: 10.1021/acsomega.3c05151. eCollection 2023 Oct 10.

Authors

Takahiko Matsushita^{1

2

3}, Momoka Nozaki¹, Mio Sunaga¹, Tetsuo Koyama¹, Ken Hatano^{1

2

3}, Koji Matsuoka^{1

2

3}

Affiliations

¹ Area for Molecular Function, Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan.
² Medical Innovation Research Unit (MiU), Advanced Institute of Innovative Technology (AIIT), Saitama University, Sakura, Saitama 338-8570, Japan.
³ Health Science and Technology Research Area, Strategic Research Center, Saitama University, Sakura, Saitama 338-8570, Japan.

Abstract

Glycomonomers having N-glycosidic linkages were prepared from a known glycosyl amine, N-acetyl-d-glucosamine (GlcNAc). Radical polymerization of the glycomonomers gave a series of glycopolymers displaying various sugar densities, which were models of the core structure of Asn-linked-type glycoproteins. In addition, fluorometric analyses of wheat germ agglutinin (WGA) against the glycopolymers were carried out, and the results showed unique binding specificities on the basis of flexibility of sugar moieties.