Association between miR-365 polymorphism and ischemic stroke in a Chinese population

Yin-Hua Weng; Wen-Tao Yu; Yan-Ping Luo; Chao Liu; Xi-Xi Gu; Huo-Ying Chen; Hong-Bo Liu

doi:10.3389/fneur.2023.1260230

Association between miR-365 polymorphism and ischemic stroke in a Chinese population

Front Neurol. 2023 Sep 27:14:1260230. doi: 10.3389/fneur.2023.1260230. eCollection 2023.

Authors

Yin-Hua Weng^#^{1

2

3}, Wen-Tao Yu^#³, Yan-Ping Luo³, Chao Liu^{1

2

3}, Xi-Xi Gu³, Huo-Ying Chen^{1

2

4}, Hong-Bo Liu^{1

2

4}

Affiliations

¹ Department of Laboratory Medicine, The Second Affiliated Hospital of Guilin Medical University, Guilin, China.
² College of Medical Laboratory Science, Guilin Medical University, Guilin, China.
³ School of Clinical Medicine, Guilin Medical University, Guilin, China.
⁴ Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, Guangxi Key Laboratory of Metabolic Reprogramming and Intelligent Medical Engineering for Chronic Diseases, The Second Affiliated Hospital of Guilin Medical University, Guilin, China.

^# Contributed equally.

Abstract

Background: Ischemic stroke (IS) represents a major cause of morbidity and mortality across the globe. The aberrant expression of miR-365 has been found to be implicated in a wide array of human diseases, including atherosclerosis and cancer. Studies on single-nucleotide polymorphisms (SNPs) in miRNA genes can help gain insight into the susceptibility to the condition. This study aimed to examine the relationship between miR-365 SNPs and the risk of IS.

Methods: The study recruited 215 IS patients and 220 controls. The SNPscans genotyping was employed to genotype three polymorphic loci (rs121224, rs30230, and rs178553) of miR-365. The relative expression of miR-365 in peripheral blood mononuclear cells of the patients and controls was determined by using real-time quantitative PCR.

Results: The miR-365 rs30230 polymorphism exhibited a significant association with the risk of developing IS (TC vs. CC: adjusted OR = 0.55, 95% CI: 0.33-0.92, P = 0.022; TT vs. CC: adjusted OR = 0.34, 95% CI: 0.14-0.85, P = 0.021; TC +TT vs. CC: adjusted OR = 0.51, 95% CI: 0.31-0.83, P = 0.007; T vs. C: adjusted OR = 0.57, 95% CI: 0.39-0.83, P = 0.004). Haplotype analysis revealed that the C-T-G haplotype was associated with a decreased risk of IS (OR = 0.68, 95% CI: 0.46-1.00, P = 0.047). Furthermore, miR-365 expression was significantly higher in IS patients than in controls (P < 0.001). Interestingly, patients with rs30230 TC or TT genotypes had lower miR-365 levels compared to their counterparts with CC genotypes (P < 0.001).

Conclusions: The miR-365 rs30230 polymorphism might bear an association with IS susceptibility in the Chinese population, and the rs30230 TC/TT genotype might be a protective factor against IS.

Keywords: genotype; ischemic stroke; miR-365; miRNA; polymorphism; risk.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The study was supported by the National Natural Science Foundation of China (No. 82160313), Guangxi Natural Science Foundation (No. 2020GXNSFDA297027), and Guangxi Medical and Health Key Discipline Construction Project.