Alterations of gut microbiota in a mouse model with partial small intestinal obstruction

Yong Wang; Minzhong Zhang; Lu Jiang; Yiming Gong; Keqiang Liu; Tian Zhang

doi:10.3389/fmicb.2023.1242650

Alterations of gut microbiota in a mouse model with partial small intestinal obstruction

Front Microbiol. 2023 Sep 28:14:1242650. doi: 10.3389/fmicb.2023.1242650. eCollection 2023.

Authors

Yong Wang^{1

2

3

4}, Minzhong Zhang⁵, Lu Jiang⁶, Yiming Gong⁵, Keqiang Liu⁶, Tian Zhang⁵

Affiliations

¹ Department of Pediatric Surgery, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
² Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangzhou, China.
³ Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangzhou, China.
⁴ Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.
⁵ Department of Pediatric Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁶ Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, China.

Abstract

Introduction: Changes in the gut microbiota of patients with partial small intestinal obstruction (PSIO) have not been widely clarified. We aimed to explore bacterial diversity in a PSIO mouse model.

Methods: A PSIO mouse model was established using male C57BL/6 mice, and feces samples from the distal ileum and ileum epithelium tissues were collected. MiSeq sequencing of the 16S rRNA gene was conducted to characterize microbiota diversity and composition. RNA sequencing for differences in transcriptomic programming of the ileum tissue was performed between the PSIO and (Control) Ctrl groups.

Results: Bacterial diversity in the PSIO group was significantly lower than that in the controls. Pseudomonadota was predominant in the feces of the PSIO group. Unclassified_Muribaculaceae (p = 0.008) and Akkermansia (p = 0.007) were more abundant in the Ctrl group than those in the PSIO group. Furthermore, Escherichia_Shigella (p = 0.008) was more predominant in the feces of the PSIO group. The Kyoto Encyclopedia of Genes and Genomes pathways related to metabolism were depleted in the PSIO group. Pathways associated with intestinal fibrosis, including extracellular matrix-receptor interaction, focal adhesion, phosphoinositide 3-kinase (PI3K)-Akt signaling pathway and transforming growth factor (TGF)-beta signaling pathway, which were enriched in ileum epithelial tissue in the PSIO group.

Conclusion: PSIO can lead to changes in the predominant intestinal bacterial groups. Depleted functional profiles of the gut microbiota were identified in the PSIO group. Functional pathways associated with intestinal fibrosis were activated by PSIO. The potential regulation by the microbiota needs to be explored in the future.

Keywords: depleted diversity; gut microbiota; intestinal fibrosis; partial small intestinal obstruction; pediatrics.