The novel lncRNA-9802/miR-1646 axis affects cell proliferation of DF-1 by regulating Bax/Bcl-2 signaling pathway

Shuo Han; Shuang Zhao; Yaolu Zhao; Mingchun Liu; Liping Han; Limei Han

doi:10.1016/j.rvsc.2023.105047

The novel lncRNA-9802/miR-1646 axis affects cell proliferation of DF-1 by regulating Bax/Bcl-2 signaling pathway

Res Vet Sci. 2023 Nov:164:105047. doi: 10.1016/j.rvsc.2023.105047. Epub 2023 Oct 12.

Authors

Shuo Han¹, Shuang Zhao², Yaolu Zhao³, Mingchun Liu⁴, Liping Han⁵, Limei Han⁶

Affiliations

¹ Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: 2020200162@stu.syau.edu.cn.
² Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: 2020220581@stu.syau.edu.cn.
³ Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: yaoluzhao@syau.edu.cn.
⁴ Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: liumingchun@syau.edu.cn.
⁵ Department of Bioscience, Changchun Normal University, Changchun 130032, China. Electronic address: hanliping@ccsfu.edu.cn.
⁶ Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: limeihan@syau.edu.cn.

PMID: 37837750
DOI: 10.1016/j.rvsc.2023.105047

Abstract

Marek's disease (MD) is a severe infectious and immunosuppressive neoplastic condition that significantly impacts the global poultry industry. Investigating the role of non-coding RNA in pathogenic mechanisms of MD virus (MDV) offers valuable insights for the effective prevention and management of MD. A higher expression of the novel lncRNA-9802 can be found in spleen tissues of MDV-infected chickens from our prior research, and there is a potential association between lncRNA-9802 and cell proliferation. In this study, we further demonstrated that over-expression of lncRNA-9802 could promote the proliferation of DF-1 cells. It has been established that lncRNA-9802 mediated its effects by binding to miR-1646, and further modulated the expression of the Bax and Bcl-2 genes. Deciphering the role of the recently discovered MD-associated lncRNA-9802/miR-1646 axis provides valuable theoretical basis for decoding the molecular mechanisms underlying MDV pathogenesis.

Keywords: Cell proliferation; Marek's disease; lncRNA-9802; miR-1646.

MeSH terms

Animals
Cell Proliferation
Chickens
Herpesvirus 2, Gallid* / genetics
Marek Disease* / genetics
MicroRNAs* / genetics
MicroRNAs* / metabolism
RNA, Long Noncoding* / genetics
Signal Transduction
bcl-2-Associated X Protein

Substances

bcl-2-Associated X Protein
MicroRNAs
RNA, Long Noncoding