CRISPR-Cas9 Direct Fusions for Improved Genome Editing via Enhanced Homologous Recombination

Tahmina Tabassum; Giovanni Pietrogrande; Michael Healy; Ernst J Wolvetang

doi:10.3390/ijms241914701

CRISPR-Cas9 Direct Fusions for Improved Genome Editing via Enhanced Homologous Recombination

Int J Mol Sci. 2023 Sep 28;24(19):14701. doi: 10.3390/ijms241914701.

Authors

Tahmina Tabassum¹, Giovanni Pietrogrande¹, Michael Healy², Ernst J Wolvetang¹

Affiliations

¹ Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
² Institute for Molecular Biosciences, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.

Abstract

DNA repair in mammalian cells involves the coordinated action of a range of complex cellular repair machinery. Our understanding of these DNA repair processes has advanced to the extent that they can be leveraged to improve the efficacy and precision of Cas9-assisted genome editing tools. Here, we review how the fusion of CRISPR-Cas9 to functional domains of proteins that directly or indirectly impact the DNA repair process can enhance genome editing. Such studies have allowed the development of diverse technologies that promote efficient gene knock-in for safer genome engineering practices.

Keywords: CRISPR-Cas9; DNA repair; gene editing; homologous recombination.

Publication types

Review

MeSH terms

Animals
CRISPR-Cas Systems* / genetics
DNA Repair / genetics
Gene Editing*
Genome
Homologous Recombination
Mammals

Grants and funding

This research and the APC were funded by The Australian Research Council, ARC DP210103401 Safer gene editing tools for Australian livestock and biotech industries.