Encapsulating potential and functional properties of exopolysaccharide from Limosilactobacillus reuteri KCTC 14626BP isolated from human breast milk

Selvakumar Vijayalakshmi; Jong-Rai Kim; Ramachandran Chelliah; Kaliyan Barathikannan; Akanksha Tyagi; Simon-Okomo Aloo; Xiuqin Chen; Pianpian Yan; Lingyue Shan; Deog-Hwan Oh

doi:10.1016/j.ijbiomac.2023.127330

Encapsulating potential and functional properties of exopolysaccharide from Limosilactobacillus reuteri KCTC 14626BP isolated from human breast milk

Int J Biol Macromol. 2023 Dec 31;253(Pt 8):127330. doi: 10.1016/j.ijbiomac.2023.127330. Epub 2023 Oct 11.

Affiliations

¹ Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South Korea; Centre Of Molecular Medicine and Diagnostics (COMManD), Department of Biochemistry, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600077, India.
² Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South Korea.
³ Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South Korea; Kangwon Institute of Inclusive Technology (KIIT), Kangwon National University, Chuncheon, South Korea.
⁴ Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South Korea; Agriculture and Life Science Research Institute, Kangwon National University, Chuncheon 24341, South Korea.
⁵ Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South Korea. Electronic address: deoghwa@kangwon.ac.kr.

PMID: 37832623
DOI: 10.1016/j.ijbiomac.2023.127330

Abstract

Exopolysaccharides (EPS) are natural, nontoxic, biocompatible and biodegradable macromolecules produced by microorganisms, including the Lactic acid bacteria, to enhance protection against environmental stress conditions. The current study focused on the encapsulation and functional efficiency of EPS produced by probiotic strains isolated from human milk. Among 27 isolates, the potential high EPS-producing strain Limosilactobacillus reuteri KCTC 14626BP was selected based on biofilm production. The structural Characterization of EPS was performed based on FTIR, NMR and functional properties were determined; further, the encapsulation efficiency of EPS was determined with caffeic acid. The results indicate that L. reuteri produced EPS major component consisting of glucose, galactose and arabinose with the ratio of (0.78:0.16: 0.05). The antioxidant efficiency of EPS-LR was determined on DPPH (60.3 %) and ABTS (48.9 %); EPS showed enhanced functional activities. The absence of toxicity was confirmed based on Caenorhabditis elegans. The EPS-loaded Caffeic acid (CA) EPS-LR indicated spherical capsules with rough surfaces, with sizes ranging from 1.39 to 6.75 μm. These findings indicate that EPS-LR can be applied as a bioactive compound and encapsulating material in food, cosmetics, and pharmaceutical industries.

Keywords: Caffeic acid; Encapsulation; Exopolysaccharides; Limosilactobacillus reuteri KCTC 14626BP.

MeSH terms

Caffeic Acids
Female
Humans
Limosilactobacillus reuteri*
Milk, Human
Polysaccharides, Bacterial* / chemistry

Substances

Polysaccharides, Bacterial
caffeic acid
Caffeic Acids