Characterization of an antimony-resistant fungus Sarocladium kiliense ZJ-1 and its potential as an antimony bio-remediator

Abstract

Antimony (Sb) is a toxic metalloid widely distributed in the natural environments. Microorganisms, especially fungi, could serve as ideal biomaterials for bioremediation of Sb-polluted soils and waters. In this study, we isolated an antimony-resistant fungus, Sarocladium kiliense ZJ-1, from a slag sample collected in Xikuangshan Sb mine in P. R. China. ZJ-1 showed an extremely high resistance to Sb, with a MIC level of > 175 mM for arsenite [Sb(Ⅲ)] and 40 mM for arsenate [Sb(V)]. Whole genomic analysis identified multiple Sb (Ⅲ)- and/or As(Ⅲ)-resistant genes on ZJ-1's genome, which may partially explain its hyper-resistance to Sb. The potential of ZJ-1 in removing Sb from Sb(Ⅲ) or Sb(V) solutions was also quantified. The average biosorption capacity of ZJ-1 for Sb(Ⅲ) and Sb(V) is 635.14 mg/g and 149.65 mg/g, respectively, in Sb aqueous solutions with an initial concentration of 2000 mg/L (16.43 mM). Besides, almost 99% of Sb(Ⅲ) in the growing system was removed with an initial concentration of 500 mg/L (4.11 mM). Furthermore, Fourier transformation infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) were used to probe the Sb adsorption mechanism on ZJ-1, and -OH, -NH₂, -COOH, C-O and C-O-C were found to be the main surface functional groups of ZJ-1 cells to adsorb Sb.

Keywords: Antimony; Antimony resistance; Biosorption; Fungi.