Volatile halocarbons (VHCs) are important trace greenhouse gases and ozone-destroying substances and play an important role in global climate change. As an important producer of VHCs, the release of VHCs by marine microalgae is affected by marine environmental factors. Microplastics are an important pollutant in the ocean; however, there are few studies on VHCs release from marine microalgae under the influence of microplastics. This study aimed to explore the effects of different concentrations of polyethylene (PE) microplastics on the growth, photosynthesis, oxidative stress, and release of VHCs by diatoms and dinoflagellates by measuring the density of algae, maximum photoquantum efficiency (Fv/Fm), reactive oxygen species (ROS), and concentration of VHCs. The results revealed that PE microplastics mainly inhibited the growth of Nitzschia closterium f. minutissima and promoted the growth of Prorocentrum donghaiense. The addition of 50 μm PE microplastics had a shielding effect on the growth of the two microalgae, resulting in the inhibition of Fv/Fm of two kinds of microalgae, and the inhibition effect of PE microplastics on P. donghaiense was more significant. Compared with that in the control group, PE microplastic stress stimulated the increase in ROS production in algal cells, which caused an oxidative stress response in these microalgae, thereby promoting the release of three types of volatile brominated halocarbons.
Keywords: Nitzschia closterium f.minutissima; Prorocentrum donghaiense; growth inhibition; microplastics; polyethylene; volatile halocarbons(VHCs).