A sensitive non-enzymatic dual-conductive biosensor for continuous glucose monitoring

Xiao Wang; Haoling Huo; Congjie Xu; Huaijun Lin; Qiwei Wang; Junjie Yang; Florian Vogel; Xiaoying Wang; Zhidan Lin; Lin Cao; Wei Li; Peng Zhang

doi:10.1016/j.aca.2023.341845

A sensitive non-enzymatic dual-conductive biosensor for continuous glucose monitoring

Anal Chim Acta. 2023 Oct 23:1279:341845. doi: 10.1016/j.aca.2023.341845. Epub 2023 Sep 23.

Authors

Xiao Wang¹, Haoling Huo¹, Congjie Xu¹, Huaijun Lin¹, Qiwei Wang¹, Junjie Yang¹, Florian Vogel¹, Xiaoying Wang², Zhidan Lin¹, Lin Cao³, Wei Li⁴, Peng Zhang⁵

Affiliations

¹ Institute of Advanced Wear & Corrosion Resistant and Functional Materials, Jinan University, Guangzhou, 510632, China.
² College of Life Science and Technology, Biomedical Engineering Department, Jinan University, Guangzhou, 510632, China.
³ Institute of Advanced Wear & Corrosion Resistant and Functional Materials, Jinan University, Guangzhou, 510632, China. Electronic address: caol93@jnu.edu.cn.
⁴ Institute of Advanced Wear & Corrosion Resistant and Functional Materials, Jinan University, Guangzhou, 510632, China. Electronic address: lwxasn@sohu.com.
⁵ Institute of Advanced Wear & Corrosion Resistant and Functional Materials, Jinan University, Guangzhou, 510632, China. Electronic address: tzhangpeng@jnu.edu.cn.

PMID: 37827656
DOI: 10.1016/j.aca.2023.341845

Abstract

Background: Diabetes and diabetic wound management have always been urgent issues for global healthcare. In the demand for blood glucose monitoring and wound management, phenylboronic acid (PBA)-based glucose biosensors are effective assistance due to their excellent glucose specificity, high sensitivity, and response stability. Nevertheless, PBA-based glucose biosensors still have challenges in terms of wide linearity and large deformation requirements. Therefore, it is necessary to develop PBA-based glucose biosensors with satisfactory mechanical properties, high response sensitivity, excellent stability, and wide linearity.

Results: In this work, a glucose-responsive PBA-based biosensor was successfully synthesized for the first time. The sensor materials exhibited excellent mechanical properties with an elongation at break reached up to 1000%, and the healing efficiency was over 90% within 30 min at 45 °C. Furthermore, the biosensor exhibited exceptional electromechanical responsiveness, stability, high sensitivity, and wide linearity due to the specificity of phenylboronic acid to glucose and the construction of a special HCNT/PEDOT:PSS dual conductive structure. In addition, the assembled biosensor displayed remarkable glucose, pH and temperature responses, exhibiting a linear response to glucose concentration range from 0.20 mM to 2.0 mM, with a sensitivity coefficient of 47.11 mA mM^-1 and regression coefficient of 0.942. Moreover, the sensor materials showed satisfactory cytocompatibility, hemocompatibility, and antibacterial properties against Escherichia coli and Staphylococcus aureus.

Significance: For the first time, a dual conductive structural glucose biosensor based on PBA-based copolymer was synthesized. In addition to excellent glucose sensitivity and response stability, the biosensor has a wide linearity range, excellent self-healing property, and satisfactory mechanical performance. As a promising substitute for non-enzymatic glucose biosensors, this new material with special structure and characteristics would also be beneficial to wound management in diabetic patients.

Keywords: Biosensor; Dual-conductive structure; Glucose response; PBA-based copolymer; Temperature and pH response.

MeSH terms

Biosensing Techniques*
Blood Glucose
Blood Glucose Self-Monitoring
Diabetes Mellitus*
Glucose
Humans

Substances

Blood Glucose
benzeneboronic acid
Glucose