Fluorescence Microscopy of Nanochannel-Confined DNA

Sriram Kk; Fredrik Persson; Joachim Fritzsche; Jason P Beech; Jonas O Tegenfeldt; Fredrik Westerlund

doi:10.1007/978-1-0716-3377-9_9

Fluorescence Microscopy of Nanochannel-Confined DNA

Methods Mol Biol. 2024:2694:175-202. doi: 10.1007/978-1-0716-3377-9_9.

Authors

Sriram Kk¹, Fredrik Persson², Joachim Fritzsche³, Jason P Beech⁴, Jonas O Tegenfeldt⁴, Fredrik Westerlund⁵

Affiliations

¹ Department of Life Sciences, Chalmers University of Technology, Gothenburg, Sweden.
² Moleculent, Stockholm, Sweden.
³ Department of Physics, Chalmers University of Technology, Gothenburg, Sweden.
⁴ NanoLund and Department of Physics, Lund University, Lund, Sweden.
⁵ Department of Life Sciences, Chalmers University of Technology, Gothenburg, Sweden. fredrik.westerlund@chalmers.se.

PMID: 37824005
DOI: 10.1007/978-1-0716-3377-9_9

Abstract

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.

Keywords: DNA; Fluorescence; Nanochannels; Single molecule.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA* / chemistry
Microscopy, Fluorescence
Nanostructures* / chemistry
Nanotechnology
Polymers

Substances

DNA
Polymers