GYRB - POLYMERASE CHAIN REACTION AND HISTOPATHOLOGIC CHARACTERISTIC FIGURE POTENTIAL FOR DETERMINING DIAGNOSIS OF TUBERCULOUS LYMPHADENITIS

Herlambang Wahyu; Mertaniasih Ni Made; Soedarsono Soedarsono; Sandhika Willy

doi:10.21010/Ajidv17i2S.3

GYRB - POLYMERASE CHAIN REACTION AND HISTOPATHOLOGIC CHARACTERISTIC FIGURE POTENTIAL FOR DETERMINING DIAGNOSIS OF TUBERCULOUS LYMPHADENITIS

Afr J Infect Dis. 2023 Aug 1;17(2 Suppl):13-18. doi: 10.21010/Ajidv17i2S.3. eCollection 2023.

Authors

Herlambang Wahyu¹, Mertaniasih Ni Made^{2

3

4}, Soedarsono Soedarsono^{5

4}, Sandhika Willy⁶

Affiliations

¹ Master Program of Tropical Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
² Department of Medical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
³ Department of Clinical Microbiology, Dr. Soetomo Academic Hospital, Surabaya, Indonesia.
⁴ Tuberculosis Laboratory, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
⁵ Sub-pulmonology of Internal Medicine, Faculty of Medicine, Hang Tuah University, Surabaya, Indonesia.
⁶ Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.

Abstract

Background: TB lymphadenitis is still a problem that needs serious treatment. In Indonesia, it was reported that 53% of TB cases were extrapulmonary tuberculosis, with the most cases being Lymphadenitis TB, 11.6%. In children, 43% of extrapulmonary tuberculosis cases are TB lymphadenitis. Diagnosis is quite difficult; a method of determining the diagnosis and appropriate comprehensive treatment is required in managing TB Lymphadenitis.

Materials and methods: In this study, 15 fine needle aspiration biopsy aspirate samples were subjected to molecular examination using the gyrB-polymerase chain reaction method and histopathological observations using the smear method with hematoxylin-eosin staining. Observation of preparations using a microscope with a magnification of 200x.

Results: The histopathological characteristics of the fine needle aspiration biopsy aspirate showed positive results in 4 out of 15 samples, with epithelioid cells arranged in a characteristic granuloma structure, necrotic debris was visible, and cells joined together to form multinucleated giant cells as an inflammatory response to Mycobacterium tuberculosis complex infection. In this study, 6 out of 15 (40%) were detected to be positive in the diagnosis based on molecular detection using a specific target gene gyrB - polymerase chain reaction .

Conclusion: Characteristic features on histopathological examination associated with gyrB - positive polymerase chain reaction on lymphadenitis fine needle aspiration biopsy aspirate samples can be used as a determinant diagnosis of tuberculous lymphadenitis.

Keywords: EPTB; FNAB aspirate; PCR; gyrB; tuberculosis; tuberculous lymphadenitis.