MiR-216a reduces apoptosis of pulmonary microvascular endothelial cells in COPD by targeting DNMT1

Ling Lin; Qing Song; Wei Cheng; Cong Liu; Aiyuan Zhou; Zijing Zhou; Ping Chen

doi:10.18332/tid/171357

MiR-216a reduces apoptosis of pulmonary microvascular endothelial cells in COPD by targeting DNMT1

Tob Induc Dis. 2023 Oct 10:21:130. doi: 10.18332/tid/171357. eCollection 2023.

Authors

Ling Lin^{1

2

3}, Qing Song^{1

2

3}, Wei Cheng^{1

2

3}, Cong Liu^{1

2

3}, Aiyuan Zhou⁴, Zijing Zhou^{1

2

3}, Ping Chen^{1

2

3}

Affiliations

¹ Department of Respiratory and Critical Care Medicine, the Second Xiangya Hospital, Central South University, Changsha, China.
² Research Unit of Respiratory Disease, Central South University, Changsha, China.
³ Diagnosis and Treatment Center of Respiratory Disease, Central South University, Changsha, China.
⁴ Department of Respiratory and Critical Care Medicine, the Xiangya Hospital, Central South University, Changsha, China.

Abstract

Introduction: Abnormal apoptosis of pulmonary microvascular endothelial cells (PMVECs) participates in the pathogenesis of COPD. Studies have shown that microRNAs (miRNAs) contribute to the pathogenesis of pulmonary diseases by regulating cell apoptosis. The present study aimed to investigate the effects of miR-216a in cigarette smoke extract (CSE)-induced apoptosis of PMVECs in COPD and explore the potential mechanisms.

Methods: The emphysema model mice were treated with CSE and CS exposure. The expression of miR-216a and DNA methyltransferase 1 (DNMT1) was assessed in emphysema mice and COPD patients. The miR-216a mimic and Lenti-DNMT1 were transfected into PMVECs to identify the underlying mechanisms. The expression levels of miR-216a and DNMT1 were detected by real-time quantitative polymerase chain reaction (RT-qPCR) or Western blot. Moreover, cell apoptosis was examined by flow cytometry assays.

Results: The results show that the expression of miR-216a was decreased, whereas the expression of DNMT1 was increased in the lung tissue of emphysema mice and COPD patients. In addition, the expression of miR-216a was significantly reduced in CSE-treated PMVECs, and the overexpression of miR-216a attenuated CSE-induced PMVEC apoptosis. Furthermore, the expression of DNMT1 was increased in the CSE-induced PMVECs and then was reduced after the overexpression of miR-216a in the CSE-stimulated PMVECs. Luciferase reporter assays confirmed the target reaction between miR-216a and DNMT1. Also, the overexpression of DNMT1 was able to reverse the anti-apoptotic effect of miR-216a in CSE-induced PMVECs.

Conclusions: The results indicate that miR-216a may play a crucial role in CSE-induced apoptosis by directly regulating its target gene DNMT1 in COPD. It provides insights into the function of MiR-216a/DNMT1 as a potential molecule in COPD.

Keywords: DNMT1; MiR-216a; apoptosis; chronic obstructive pulmonary disease; pulmonary microvascular endothelial cells.