Effect of active irrigation using shock wave-enhanced emission photoacoustic streaming on dental pulp stem cell viability

Pouyan Razavi; Sohrab Tour Savadkouhi; Nooshin Barikrow; Aryan Jafari; Mehdi Vatanpour

Effect of active irrigation using shock wave-enhanced emission photoacoustic streaming on dental pulp stem cell viability

Dent Res J (Isfahan). 2023 Aug 28:20:91. eCollection 2023.

Authors

Pouyan Razavi¹, Sohrab Tour Savadkouhi², Nooshin Barikrow³, Aryan Jafari¹, Mehdi Vatanpour⁴

Affiliations

¹ Student Research Committee, Dental School, Islamic Azad University of Medical Sciences, Tehran, Iran.
² Dental Material Research Center, Dental School, Islamic Azad University of Medical Sciences, Tehran, Iran.
³ Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
⁴ Department of Endodontic, Dental School, Islamic Azad University of Medical Sciences, Tehran, Iran.

PMID: 37810454
PMCID: PMC10557991

Abstract

Background: Shock wave-enhanced emission photoacoustic streaming (SWEEPS) is a novel irrigation activation method based on photoacoustic streaming. The aim of this study was to look into the impact of SWEEPS on the attachment and survival of dental pulp stem cells (DPSCs).

Materials and methods: In this in vitro study, 34 standardized root segments were randomly allocated into two groups: SWEEPS and the conventional conditioning group. After the irrigation, human DPSCs were seeded on the internal walls of these samples, and the attachment and survival of 30 of them were assessed on different days. The remaining two samples were observed using a scanning electron microscope (SEM). Independent sample t-test, Mann-Whitney U-test, one-way ANOVA, Kruskal-Wallis, and two-way ANOVA were used for data analysis with the level of significance = 0.05.

Results: The viability of DPSCs was significantly greater in the SWEEPS group in comparison with the conventional conditioning group (P = 0.029). Both groups have shown a significant increase in the viability of DPSCs over time (P = 0.0001, P = 0.003). SEM results have shown a smear layer-free surface with firmly attached DPSCs in the SWEEPS group.

Conclusion: The results of this study indicated that active irrigation using SWEEPS could provide a superior surface in terms of viability and attachment of DPSCs compared to the conventional conditioning method.

Keywords: Lasers; mesenchymal stem cells; regenerative endodontics; root canal irrigants; smear layer.