Alcohol drinking alters oral microbiota to modulate the progression of alcohol-related liver disease

Chuyue Pan; Chang Liu; Wenxin Jia; Danyang Zhao; Xiaoshan Chen; Xiang Zhu; Maohui Yang; Lirui Wang

doi:10.1016/j.isci.2023.107977

Alcohol drinking alters oral microbiota to modulate the progression of alcohol-related liver disease

iScience. 2023 Sep 19;26(10):107977. doi: 10.1016/j.isci.2023.107977. eCollection 2023 Oct 20.

Authors

Chuyue Pan¹, Chang Liu², Wenxin Jia¹, Danyang Zhao¹, Xiaoshan Chen¹, Xiang Zhu¹, Maohui Yang², Lirui Wang²

Affiliations

¹ School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiang Su 211198, China.
² Institute of Modern Biology, Nanjing University, Nanjing 210008, China.

Abstract

Alcohol-related liver disease (ALD) is one of the leading causes of liver-related death worldwide. However, roles of oral microbiota in regulating the progression of ALD remain unknown. Here, we fed mice with control or ethanol diet to establish chronic-plus-binge ALD model. 16S ribosomal DNA sequencing was performed on oral and cecum samples. We demonstrated that alcohol drinking influenced bacterial richness, microbial structure, and composition in oral samples of ethanol-fed mice compared with control mice. Alcohol consumption also remodeled relationships among oral microbes and altered functions of oral microbiota. Furthermore, oral microbiota, such as Streptococcus, Helicobacter, Alloprevotella, and Psychrobacter were closely associated with ALD parameters. Finally, we observed Sutterellaceae_uncultured, Dyella, and Gemmatimonas possibly translocated along with oral-gut axis and positively correlated with the severity of ALD. Altogether, alcohol consumption reprogramed composition and functions of oral microbiota to promote ALD progression, suggesting that oral microbes might become a new target for ALD therapy.

Keywords: Health sciences; Medicine; Microbiology.