SARS-CoV-2 spike protein-derived immunogenic peptides that are promiscuously presented by several HLA-class II molecules and their potential for inducing acquired immunity

Yuki Yajima; Akemi Kosaka; Takayuki Ohkuri; Yoshihiko Hirohashi; Dongliang Li; Takeshi Nagasaki; Toshihiro Nagato; Toshihiko Torigoe; Hiroya Kobayashi

doi:10.1016/j.heliyon.2023.e20192

SARS-CoV-2 spike protein-derived immunogenic peptides that are promiscuously presented by several HLA-class II molecules and their potential for inducing acquired immunity

Heliyon. 2023 Sep 20;9(9):e20192. doi: 10.1016/j.heliyon.2023.e20192. eCollection 2023 Sep.

Authors

Yuki Yajima^{1

2}, Akemi Kosaka², Takayuki Ohkuri², Yoshihiko Hirohashi³, Dongliang Li⁴, Takeshi Nagasaki⁴, Toshihiro Nagato², Toshihiko Torigoe³, Hiroya Kobayashi²

Affiliations

¹ Department of Oral and Maxillofacial Surgery, Asahikawa Medical University, Asahikawa, Hokkaido, Japan.
² Department of Pathology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan.
³ Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
⁴ Tsukuba Laboratory, Medical & Biological Laboratories Co., Ltd., Ina, Japan.

Abstract

The current coronavirus disease 2019 (COVID-19) pandemic that is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has a significant threat to public health. Although vaccines based on the mRNA of the SARS-CoV-2 spike protein have been developed to induce both cellular and humoral immunity against SARS-CoV-2, there have been some concerns raised about their high cost, particularly in developing countries. In the present study, we aim to identify an immunogenic peptide in the SARS-CoV-2 spike protein to activate cellular immunity, particularly CD4⁺ helper T lymphocytes (Th cells), which are a commander of immune system. SARS-CoV-2 spike protein-derived peptides Spike_448-477 and Spike_{489-513(N501Y)}-specific CD4⁺ Th cell lines were generated by repetitive stimulation of healthy donor-derived CD4⁺T-cells with each peptide. Their HLA-restrictions were addressed by using blocking antibodies against HLA and HLA-transfected L-cells. The epitopes of Spike_448-477-specific CD4⁺ Th cell lines were defined using a series of 7-14-mer overlapping truncated peptides and alanine-substituted epitope peptides. To address responsiveness of these CD4⁺ Th cell lines to several SARS-CoV-2 variants, we stimulated the CD4⁺ Th cell lines with mutated peptides. We addressed whether these identified peptides were useful for monitoring T-cell-based immune responses in vaccinated donors using the IFN-γ ELISpot assay. The Spike_448-477 peptide was found to be a promiscuous peptide presented by HLA- DRB1*08:02, DR53, and DPB1*02:02. Although HLA-DPB1*02:02-restricted CD4⁺ Th cells did not response to some peptides with the L452R and L452Q mutations, the other CD4⁺ Th cells were not affected by any mutant peptides. We developed two tetramers to detect HLA-DRB1*08:02/Spike_449-463- and Spike_449-463(L452R/Y453F)-recognizing CD4⁺ Th cells. Spike_{489-513(N501Y)} peptide was also a promiscuously presented to HLA-DRB1*09:01 and DRB1*15:02. The T-cell responses specific to both peptides Spike_448-477 and Spike_489-513 were detected in PBMCs after vaccinations. In addition, we observed that the Spike_448-477 peptide activated both CD8⁺ T-cells and CD4⁺ Th cells in individuals receiving mRNA vaccines. SARS-CoV-2 spike protein-derived peptides, Spike_448-477 and Spike_489-513, include several epitopes that are presented by multiple HLA-class II alleles to activate CD4⁺ Th cells, which are considered useful for monitoring the establishment of acquired immunity after vaccination.

Keywords: Cellular immunity; Helper T lymphocytes; SARS-CoV-2; T-cell monitoring.