Evaluating phytochemical constituents and in-vitro antiplasmodial and antioxidant activities of Fadogiella stigmatoloba, Hygrophylla auriculata, Hylodesmum repandum, and Porphyrostemma chevalieri extracts

Henry Manya Mboni; Marie Faes; Stéphanie Fraselle; Moussa Compaoré; Bakari Amuri Salvius; Kahumba Byanga Joseph; Pierre Duez; Lumbu Simbi Jean-Baptiste; Caroline Stévigny

doi:10.1016/j.heliyon.2023.e20103

Evaluating phytochemical constituents and in-vitro antiplasmodial and antioxidant activities of Fadogiella stigmatoloba, Hygrophylla auriculata, Hylodesmum repandum, and Porphyrostemma chevalieri extracts

Heliyon. 2023 Sep 13;9(9):e20103. doi: 10.1016/j.heliyon.2023.e20103. eCollection 2023 Sep.

Authors

Henry Manya Mboni^{1

2}, Marie Faes², Stéphanie Fraselle², Moussa Compaoré³, Bakari Amuri Salvius¹, Kahumba Byanga Joseph¹, Pierre Duez⁴, Lumbu Simbi Jean-Baptiste⁵, Caroline Stévigny²

Affiliations

¹ Laboratoire de Pharmacognosie, Faculté des Sciences Pharmaceutiques, Université de Lubumbashi, BP.1825, Lubumbashi, Democratic Republic of the Congo.
² Unité de Pharmacognosie, Bioanalyse et Médicaments, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Campus de la Plaine - CP205/9, Boulevard du Triomphe, B-1050, Bruxelles, Belgium.
³ Laboratoire de Biochimie et Chimie Appliquée, Université Ouaga I Pr Joseph KI-ERBO, 03 BP. 7021, Ouagadougou 03, Burkina Faso.
⁴ Service de Chimie Thérapeutique et de Pharmacognosie, Université de Mons (UMONS), 20 Place du Parc, 7000, Mons, Belgium.
⁵ Service de Chimie Organique, Département de Chimie, Faculté des Sciences, Université de Lubumbashi, BP. 1825, Lubumbashi, Democratic Republic of the Congo.

Abstract

Introduction: Fadogiella stigmatoloba, Hygrophylla auriculata, Hylodesmum repandum and Porphyrostemma chevalieri are used against malaria in traditional medicine in the Democratic Republic of the Congo (DRC). To evaluate their potential in the treatment of this disease, the in vitro antiplasmodial property of these four plants was evaluated. All experiments were conducted on methanolic extracts performed on selected organ parts of these plants.

Methods: The methanolic extracts, obtained by maceration, were firstly screened in vitro against the chloroquine sensitive (3D7) and resistant (W2) Plasmodium falciparum strains by the measurement of lactate dehydrogenase activity, and on human keratinocytes (HaCat) cells by the MTT assay to determine their selectivity indices (SI). Secondly, the antioxidant activity of the same extracts was evaluated using DPPH and FRAP assays. Finally, the presence of specific phytochemical constituents was evaluated using standard methods and tentatively identified by GC-MS.

Results: An optimum antiplasmodial activity (IC₅₀ = 3.4 ± 0.7 μg/mL, for 3D7, SI = 58.2; IC₅₀ = 7 ± 1.0 μg/mL, for W2, SI = 28.3) was obtained with the leave extract of P. chevalieri. The leaves (for F. stigmatoloba and H. repandum), and the aerial part (for H. repandum) extracts showed promising and moderate antiplasmodial activities against respectively the 3D7 strain (IC₅₀: <15 μg/mL), and W2 strain (IC₅₀:15-50 μg/mL). All extracts presented a weak cytotoxic effect (IC₅₀: >100 μg/mL) on HaCat cells. For the antioxidant test, the most interesting activity was obtained with the leaf extract of P. chevalieri. The GC-MS analysis of these four plants species extracts revealed the presence of various compounds, such as Ethyl 2-nonenoate, 2-(2-Hydroxy-2-phenylethyl)-3,5,6-trimethyl pyrazine, Palmitic Acid, Ethyl palmitate, Ethyl linolenate, and N-Acetyltyramine.

Conclusion: Based on the obtained results, P. chevalieri could be selected for further investigations or ⁄and for the management of malaria after standardization.

Keywords: Antioxidant activity; Antiplasmodial activity; Cytotoxicity; Phytochemical screening; Plasmodium falciparum; Porphyrostemma chevalieri.