Diversity of Bacteroidaceae family in gut microbiota of patients with chronic kidney disease and end stage renal disease

Siamak Amini Khiabani; Setareh Haghighat; Hamid Tayebi Khosroshahi; Mohammad Asgharzadeh; Hossein Samadi Kafil

doi:10.34172/hpp.2023.29

Diversity of Bacteroidaceae family in gut microbiota of patients with chronic kidney disease and end stage renal disease

Health Promot Perspect. 2023 Sep 11;13(3):237-242. doi: 10.34172/hpp.2023.29. eCollection 2023.

Authors

Siamak Amini Khiabani¹, Setareh Haghighat¹, Hamid Tayebi Khosroshahi², Mohammad Asgharzadeh³, Hossein Samadi Kafil⁴

Affiliations

¹ Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
² Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
³ Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁴ Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Background: Human intestine microbiota are known to be directly and indirectly altered during some diseases such as kidney complications. Bacteroides is considered as the main and the most abundant phylum among human gut microbiota, which has been classified as enterotype 1. This study aimed to assess the abundance of Bacteroides spp. in fecal flora of end-stage renal disease (ESRD) and chronic kidney disease (CKD) patients and compare it with the Bacteroides composition among fecal flora of healthy individual.

Methods: Fresh fecal samples were collected from 20 CKD/ESRD patients and 20 healthy individual without any kidney complications. The pure microbial DNA was extracted by QIAamp Stool Mini Kit from stool samples. MiSeq system was used to analyze the intestinal composition by next generation sequencing method.

Results: A number of 651 bacterial strains were isolated and identified from 40 fecal samples of both patients and healthy groups. Bioinformatics analysis defined 18 different types of Bacteroides species which included 2.76% of all strains. Statistical analysis showed no significant difference between study groups (P>0.05). In both healthy and patient groups three species including B. dorei, B. uniformis, and B. ovatus have allocated the most abundance to themselves. The lowest abundance was related to B. eggerthii, A. furcosa and B. barnesiae among CKD/ESRD patients and A. furcosa, B. barnesiae, and B. coprocola had the lowest abundance among healthy people.

Conclusion: This study indicates despite all previous evidence of Bacteroides role in gut microbiota, it had no different distribution between healthy persons and CKD/ESRD patients.

Keywords: Bacteroidaceae; Chronic kidney disease; End-stage renal disease; Next generation sequencing.