Deep-brain optical recording of neural dynamics during behavior

Zhe Charles Zhou; Adam Gordon-Fennell; Sean C Piantadosi; Na Ji; Spencer LaVere Smith; Michael R Bruchas; Garret D Stuber

doi:10.1016/j.neuron.2023.09.006

Deep-brain optical recording of neural dynamics during behavior

Neuron. 2023 Dec 6;111(23):3716-3738. doi: 10.1016/j.neuron.2023.09.006. Epub 2023 Oct 6.

Authors

Zhe Charles Zhou¹, Adam Gordon-Fennell¹, Sean C Piantadosi¹, Na Ji², Spencer LaVere Smith³, Michael R Bruchas⁴, Garret D Stuber⁵

Affiliations

¹ Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA 98195, USA; Center for Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA 98195, USA.
² Department of Physics, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
³ Department of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara, CA 93106, USA.
⁴ Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA 98195, USA; Center for Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA 98195, USA; Department of Pharmacology, University of Washington, Seattle, WA 98195, USA; Department of Bioengineering, University of Washington, Seattle, WA 98195, USA. Electronic address: mbruchas@uw.edu.
⁵ Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA 98195, USA; Center for Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA 98195, USA; Department of Pharmacology, University of Washington, Seattle, WA 98195, USA. Electronic address: gstuber@uw.edu.

PMID: 37804833
PMCID: PMC10843303 (available on 2024-12-06)
DOI: 10.1016/j.neuron.2023.09.006

Abstract

In vivo fluorescence recording techniques have produced landmark discoveries in neuroscience, providing insight into how single cell and circuit-level computations mediate sensory processing and generate complex behaviors. While much attention has been given to recording from cortical brain regions, deep-brain fluorescence recording is more complex because it requires additional measures to gain optical access to harder to reach brain nuclei. Here we discuss detailed considerations and tradeoffs regarding deep-brain fluorescence recording techniques and provide a comprehensive guide for all major steps involved, from project planning to data analysis. The goal is to impart guidance for new and experienced investigators seeking to use in vivo deep fluorescence optical recordings in awake, behaving rodent models.

Deep-brain optical recording of neural dynamics during behavior

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