In vitro anticholinergic and antiglycaemic properties of frost-hardy Actinidia fruit extracts and their polyphenol profile, L-ascorbic acid content and antioxidant capacity

Food Res Int. 2023 Nov;173(Pt 1):113324. doi: 10.1016/j.foodres.2023.113324. Epub 2023 Jul 26.

Abstract

The aim of this study was to investigate the inhibitory effects of Actinidia arguta ('Weiki', 'Skarlet September Kiwi') and Actinidia kolomikta ('Lande') fruit extracts against advanced glycation end-products (AGEs) formation and acetylcholinesterase (AChE) activity. The extracts were also tested regarding polyphenol profile and Lascorbic acid content (UHPLC-DAD-MS), and antioxidant capacity (DPPH, ABTS). 'Scarlet September Kiwi' showed the strongest anti-AGEs activity studied with BSAGLU (IC50 = 2.68) and BSA-MGO (IC50 = 18.06) models. The highest anti-AChE activity was found for the 'Lande' extract (IC50 = 4.56). 'Lande' showed the highest L-ascorbic acid content (8271.96 µg/g dw), ABTS (312.42 µmol TE/g dw) and DPPH (282.01 µmol TE/g dw) values. 'Scarlet September Kiwi' revealed the highest individual phenolics concentration (2321.43 µg/g dw). The contents of (+)-catechin and L-ascorbic acid were significantly correlated with anti-AChE activity. This research sheds new light on the bioactivity of Actinidia arguta and Actinidia kolomikta fruit elucidating the role of (+)-catechin and L-ascorbic acid in prevention of Alzheimer's disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholinesterase
  • Actinidia* / chemistry
  • Antioxidants / analysis
  • Ascorbic Acid / analysis
  • Catechin* / analysis
  • Cholinergic Antagonists / analysis
  • Fruit / chemistry
  • Plant Extracts / chemistry
  • Polyphenols / analysis
  • Polyphenols / pharmacology

Substances

  • Antioxidants
  • Polyphenols
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Catechin
  • Cholinergic Antagonists
  • Acetylcholinesterase
  • Plant Extracts
  • Ascorbic Acid