Transcriptome analysis of osteogenic differentiation of human maxillary sinus mesenchymal stem cells using RNA-Seq

Yutao Zhou; Rui Jiang; Jindi Zeng; Yu Chen; Jing Ren; Songling Chen; Ermin Nie

doi:10.1016/j.heliyon.2023.e20305

Transcriptome analysis of osteogenic differentiation of human maxillary sinus mesenchymal stem cells using RNA-Seq

Heliyon. 2023 Sep 25;9(10):e20305. doi: 10.1016/j.heliyon.2023.e20305. eCollection 2023 Oct.

Authors

Yutao Zhou¹, Rui Jiang², Jindi Zeng³, Yu Chen², Jing Ren², Songling Chen², Ermin Nie²

Affiliations

¹ Department of Stomatology, Panyu Central Hospital, Guangzhou, China.
² Department of Stomatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
³ Department of Stomatology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Abstract

Recent studies have demonstrated that human maxillary sinus mesenchymal stem cells (hMSMSCs) have osteogenic potential and can be osteogenically induced. Here, we investigated pivotal molecular functions and candidates that contribute to the osteogenic differentiation of hMSMSCs. Human maxillary sinus membranes were harvested from 3 patients with jaw deformities. hMSMSCs from human maxillary sinus membranes were osteogenically induced for 0 or 21 days. Subsequently, their functional profiles were analysed by RNA sequencing and validated by quantitative PCR. Compared with control hMSMSCs, osteogenically induced hMSMSCs showed (1) osteogenic differentiation phenotype, as evidenced by the cell nodes, alizarin red staining, osteogenesis-related protein, and RNA expression; (2) accelerated osteogenic process of ossification and calcium signalling, as demonstrated by Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway; (3) enriched osteogenesis gene expression of SMOC2, OMD, IGF1, JUNB, BMP5, ADRA1A, and IGF2, which was validated by quantitative PCR. Based on by these results, we demonstrated that accelerated ossification process, calcium signalling, and upregulation of SMOC2, OMD, IGF1, JUNB, BMP5, ADRA1A and IGF2, may contribute to the osteogenic differentiation of hMSMSCs.

Keywords: Dental implantation; Human maxillary sinus mesenchymal stem cells; Osteogenic differentiation; RNA-Seq; Schneiderian membrane.