Efficient ilamycins production utilizing Enteromorpha prolifera by metabolically engineered Streptomyces atratus

Yu-Xi Jiang; Gao-Fan Zheng; Long-Chao Chen; Na Yang; Xiu-Juan Xin; Jun-Ying Ma; Jian-Hua Ju; Hui Wu; Ming Zhao; Ruida Wang; Fa-Liang An

doi:10.1186/s13068-023-02398-w

Efficient ilamycins production utilizing Enteromorpha prolifera by metabolically engineered Streptomyces atratus

Biotechnol Biofuels Bioprod. 2023 Oct 5;16(1):151. doi: 10.1186/s13068-023-02398-w.

Authors

Yu-Xi Jiang^#¹, Gao-Fan Zheng^#¹, Long-Chao Chen¹, Na Yang¹, Xiu-Juan Xin¹, Jun-Ying Ma², Jian-Hua Ju², Hui Wu¹, Ming Zhao³, Ruida Wang^{4

5}, Fa-Liang An^{6

7}

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
² CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 528225, China.
³ Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, College of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, 241000, China.
⁴ State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China. biord@mail.ecust.edu.cn.
⁵ Department of Applied Biology, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China. biord@mail.ecust.edu.cn.
⁶ State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China. flan2016@ecust.edu.cn.
⁷ Marine Biomedical Science and Technology Innovation Platform of Lin-Gang Special Area, No.4, Lane 218, Haiji Sixth Road, Shanghai, 201306, China. flan2016@ecust.edu.cn.

^# Contributed equally.

Abstract

With the invasion of green tides and the increase of urban green areas worldwide, multimillion tons of Enteromorpha need to be reutilized. In this study, Enteromorpha prolifera powder is considered a promising biomass resource for the production of commercial chemical products production. Ilamycins, novel cyclic heptapeptides with significant anti-TB activities, are isolated from Streptomyces atratus SCSIO ZH16, a deep-sea-derived strain. Using EP powder as a nitrogen source, the production of ilamycins reached 709.97 mg/L through optimization of the nitrogen source using the engineered strain S. atratus SCSIO ZH16 ΔR. After mutant strain constructions and tests, strain S. atratus SCSIO ZH16 ΔR::bldD EP powder achieved a higher production titer of ilamycins. Furthermore, the production titer of ilamycins and ilamycin E reached 1561.77 mg/L and 745.44 mg/L, respectively, in a 5 L bioreactor. This study suggests that E. prolifera is a promising and eco-friendly nitrogen source for the production of ilamycins.

Keywords: Anti-tuberculosis; BldD; Entromorpha prolifera; Ilamycins; Streptomyces atratus SCSIO ZH16.

Abstract

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