Involvement of a silkworm D2-like dopamine receptor in the promotion of feeding and related behaviors

Abstract

The biogenic amine dopamine (DA) regulates various physiological and behavioral processes in insects through binding with specific receptors. Three types of typical receptors are known to date. Previously, we achieved functional and pharmacological characterization of the three DA receptors in the silkworm Bombyx mori (BmDopR1-3). BmDopR1 and BmDopR2 are functionally classified as D1-like DA receptors, and BmDopR3 as D2-like. The present pharmacological data and our previous studies suggested that bromocriptine (Bro), which acts as an agonist on the DA D2 receptors and also interacts with various serotonin and adrenergic receptors in vertebrates, is an agonist that also acts specifically on BmDopR3, with little effect on BmDopR1 and BmDopR2 in silkworms. Exploiting this subtype specificity of Bro, to offer clues on the involvement of DA and its receptors in silkworm feeding behavior, Bro was injected into fifth instar larvae and subsequent feeding and related behaviors (feeding amount, excretion amount, mandibular movement, and feeding behavior observation) were quantitatively evaluated. Bro injection increased feeding and excretion amounts but did not affect mandibular chewing speed. Visual observation of feeding behavior for 1 h revealed the prolongation of feeding and related moving time in Bro-injected larvae. Collectively, these results suggest that Bro directly acted on BmDopR3 as an agonist and promoted feeding and related behaviors in silkworm larvae.

Keywords: Bombyx mori; Bromocriptine; Dopamine; Feeding behavior; G protein-coupled receptor (GPCR); cAMP.