Objective: The differential gene analysis of ferroptosis inreating allergic rhinitis with Bu-Zhong-Yi-Qi-Decoction based on GEO using network pharmacology and molecular docking .
Method: This study used databases such as TCMSP to search for traditional Chinese herbal medicine's active ingredients and targets in Bu-Zhong-Yi-Qi-Decoction in treating allergic rhinitis. GeneCards, OMIM, TTD, and PharmaGkb were used to obtain disease targets for allergic rhinitis, and R language was used to screen Bu-Zhong-Yi-Qi-Decoction as the main target for treating allergic rhinitis. Retrieve the gene dataset of allergic rhinitis using the GEO database, analyze ferroptosis-related genes, and select the intersection of effective targets of Bu-Zhong-Yi-Qi-Decoction for treating allergic rhinitis and ferroptosis-related genes of allergic rhinitis, draw protein interaction networks using the STRING database, use Cytoscape software to construct the target regulatory network of Bu-Zhong-Yi-Qi-Decoction for treating allergic rhinitis and ferroptosis related genes, and then use the CytoNCA plugin to screen key targets. Using R language, Gene ontology, and the biological pathway enrichment analysis were performed on the predicted targets related to the treatment of allergic rhinitis and ferroptosis with Bu-Zhong-Yi-Qi-Decoction. Selecting key targets and active ingredients for molecular docking to explore the potential mechanism of Bu-Zhong-Yi-Qi-Decoction in treating ferroptosis in allergic rhinitis.
Result: After searching the TCMSP database, a total of 182 active ingredients were obtained from 8 traditional Chinese medicines of Bu-Zhong-Yi-Qi-Decoction, such as naringenin, kaempferol, Isorhamnetin, corresponding to 3023 targets and 2025 targets related to allergic rhinitis. There are 30 remarkably enriched Go analyses for biological function of potential target genes of Bu-Zhong-Yi-Qi-Decoction in allergic rhinitis, such as regulation of apoptotic signaling pathway, cellular response to peptide, wound healing, etc. Among them, there are 7 key genes related to the treatment of allergic rhinitis and ferroptosis with Bu-Zhong-Yi-Qi-Decoction, namely TP53, MAPK1, MAPK14, HIF1A, AR, CAV1, GSK3B.
Conclusion: The treatment of allergic rhinitis with Bu-Zhong-Yi-Qi-Decoction is a process involving multiple divisions, targets, and pathways. These results indicated that oral Bu-Zhong-Yi-Qi-Decoction may effectively treat allergic rhinitis in clinical practice.