In an era of climate change and increased environmental variability, breeders are looking for tools to maintain and increase genetic gain and overall efficiency. In recent years the field of high throughput phenotyping (HTP) has received increased attention as an option to meet this need. There are many platform options in HTP, but ground-based handheld and remote aerial systems are two popular options. While many HTP setups have similar specifications, it is not always clear if data from different systems can be treated interchangeably. In this research, we evaluated two handheld radiometer platforms, Cropscan MSR16R and Spectra Vista Corp (SVC) HR-1024i, as well as a UAS-based system with a Sentera Quad Multispectral Sensor. Each handheld radiometer was used for two years simultaneously with the unoccupied aircraft systems (UAS) in collecting winter wheat breeding trials between 2018-2021. Spectral reflectance indices (SRI) were calculated for each system. SRI heritability and correlation were analyzed in evaluating the platform and SRI usability for breeding applications. Correlations of SRIs were low against UAS SRI and grain yield while using the Cropscan system in 2018 and 2019. Dissimilarly, the SVC system in 2020 and 2021 produced moderate correlations across UAS SRI and grain yield. UAS SRI were consistently more heritable, with broad-sense heritability ranging from 0.58 to 0.80. Data standardization and collection windows are important to consider in ensuring reliable data. Furthermore, practical aspects and best practices for these HTP platforms, relative to applied breeding applications, are highlighted and discussed. The findings of this study can be a framework to build upon when considering the implementation of HTP technology in an applied breeding program.
Keywords: field based phenotyping; high throughput phenotyping; plant breeding; remote sensing; spectral reflectance indices; unoccupied aircraft system - UAS.
Copyright © 2023 Herr and Carter.