Mechanoresponses mediated by the TRP11 channel in cilia of Chlamydomonas reinhardtii

Daichi Oshima; Megumi Yoshida; Kosuke Saga; Neo Ito; Miyu Tsuji; Atsuko Isu; Nobuo Watanabe; Ken-Ichi Wakabayashi; Kenjiro Yoshimura

doi:10.1016/j.isci.2023.107926

Mechanoresponses mediated by the TRP11 channel in cilia of Chlamydomonas reinhardtii

iScience. 2023 Sep 15;26(10):107926. doi: 10.1016/j.isci.2023.107926. eCollection 2023 Oct 20.

Authors

Daichi Oshima¹, Megumi Yoshida¹, Kosuke Saga¹, Neo Ito¹, Miyu Tsuji¹, Atsuko Isu², Nobuo Watanabe³, Ken-Ichi Wakabayashi^{2

4}, Kenjiro Yoshimura¹

Affiliations

¹ Department of Machinery and Control Systems, College of Systems Engineering and Science, Shibaura Institute of Technology, Saitama 337-8570, Japan.
² Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan.
³ Department of Bio-Science and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, Saitama 337-8570, Japan.
⁴ Department of Industrial Life Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Kyoto 603-8555, Japan.

Abstract

Cilia are organelles involved in motility and sensory transduction, but how these two functions coexist has not been elucidated in depth. Here, the involvement of the ciliary transient receptor potential (TRP) channel TRP11 in mechanoresponses is studied in Chlamydomonas reinhardtii using a TRP11-knockout mutant. The mutant has defects in the conversion of the bending mode of the cilium from forward to reverse when tapped with a glass rod, the detachment of cilia when shear is applied, the increase in ciliary beat frequency upon application of mechanical agitation by vortex mixing, and the initiation of gliding while both cilia are attached in opposite directions to a glass surface. These observations indicate that TRP11 can perceive mechanical stimuli with distinct intensities and durations and induce various types of ciliary responses.

Keywords: Microbial physiology; Microbiology.